与辣椒抗TMV L^(3)基因连锁的分子标记的筛选及种质资源抗TMV鉴定  

Application of Tobamovirus Resistance Gene L^(3)-Linked Markers in Pepper(Capsicum spp)Resources

作　　者：陈灵芝[1] 张茹[1] 王兰兰[1] 高彦萍 CHEN Lingzhi;ZHANG Ru;WANG Lanlan;GAO Yanping(Institute of Vegetables,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Potato Seed Seedling Virus Detection and Evaluation Engineering of Technplogy Research Center,Lanzhou 730070,China)

机构地区：[1]甘肃省农业科学院蔬菜研究所,兰州730070 [2]甘肃省马铃薯脱毒种薯(种苗)病毒检测及安全评价工程技术研究中心,兰州730070

出　　处：《西北农业学报》2023年第4期585-592,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃省农业科学院生物育种项目(2020GAAS09);甘肃省自然科学基金(21JR1RA356)。

摘　　要：以含抗辣椒TMV的L^(3)基因的材料L15为基础,利用与L^(3)基因连锁的3个分子标记189D23MF、PMFR11和PMFR21对种质资源进行鉴定.对L15、3份感病材料H8/H9/H11×L15 F_(1)、46份辣椒种质资源进行室内人工TMV接种并对其进行表型抗性鉴定和RT-PCR检测.对筛选出的标记在F_(1)及BC_(1)P_(1)群体中进行验证.结果表明:分子标记189D23MF在L15和12份辣椒种质上均未扩增出条带;SCAR标记PMFR11在L15和46份辣椒种质上呈现共显性分离,SCAR标记PMFR21为显性标记.通过室内人工接种鉴定方式,2份材料L15、H11×L15表现为高抗.H9×L15表现为抗,H8×L15及5份材料A1、A35、A39、A55、L16表现为中抗,11份材料表现为感病,30份材料表现为高感.在F1及BC1P1群体中PCR扩增结果表明SCAR标记PMFR21可用于辣椒抗TMV分子标记辅助选择.Based on material L15 which contained peper TMV resistance gene L^(3),and the pepper(Capsicum spp)germplasm resources were identified by three molecular markers associated with ge­netic linkage L3,which included 189D23MF.PMFR11 and PMFR21.L15,three infected materials H8/H9 H11×L15 F_(1).and 46 accesions of pepper germplasms were artificially indoors inoculated with MTV,and its henotypic resistance was identified and detected by RT-PCR.The results showed that marker 189D23MF didn’t amplified any hands on L15 and 12 collections.PMFR1l amplified a 270 bp hand on L15,280 bp on 46 collections,and resistant and susceptiahle hand differentiated clearly.PMFR21 amplified a single luminous and specific 200 bp hand on L15.and there were no such hand on 46 collections.L15 was highly resistant to TMV,and it's F_(1)was resistant and moderately resistant.The common pepper resources were short of resistant TMV genes.In F_(1)and BC_(1)P_(1)population.PMFR21 can he efficiently used for marker-assisted selection(MAS)of pepper breeding for resistance to TMV.

关 键 词：辣椒 L^(3)基因 分子标记 种质资源 TMV 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]