白花蛇舌草注射液对食管癌细胞放疗增敏的作用及机制探讨  

Effects of Hedyotis diffusa injection on the radiosensitization of esophageal cancer cells and related mechanism

作　　者：韩枫 穆明晨 郭加友 崔震庭 马建新[1] HAN Feng;MU Mingchen;GUO Jiayou;CUI Zhenting;MA Jianxin(Department of Oncology and Radiotherapy,Lianyungang Dongfang Hospital,Lianyungang,Jiangsu 222042,China)

机构地区：[1]连云港市东方医院肿瘤放疗科,江苏连云港222042

出　　处：《徐州医科大学学报》2023年第1期65-69,共5页Journal of Xuzhou Medical University

摘　　要：目的探讨白花蛇舌草注射液(HDI)对食管癌ECA-109和TE-10细胞放疗增敏的作用及其机制。方法采用CCK-8法检测ECA-109和TE-10细胞经不同浓度HDI处理24 h和48 h后的存活率。采用克隆形成实验评估HCI对ECA-109和TE-10细胞克隆形成能力的影响。将ECA-109和TE-10细胞分为不同组别,检测细胞凋亡及MAPK通路相关蛋白表达的变化。结果HDI可时间剂量依赖性地抑制ECA-109和TE-10细胞的存活率,ECA-109细胞和TE-10细胞的24 h半数抑制最大浓度(IC_(50))分别为66.56 ml/L和56.52 ml/L。HDI抑制ECA-109和TE-10细胞的克隆形成,并呈现剂量依赖关系。与单纯射线处理相比,HDI联合射线处理后ECA-109细胞和TE-10细胞的凋亡率显著增加(P<0.05),并具有剂量依赖性。与单纯射线或HDI处理相比,HDI联合放射处理后细胞中ERK、JNK、P38蛋白表达及其磷酸化水平均显著降低(P<0.05)。结论HDI能有效提高食管癌细胞的放疗敏感性,这可能与降低MAPK通路中相关蛋白ERK、JNK、P38的磷酸化水平有关。Objective To investigate the radiosensitizing effect of Hedyotis diffusa(HDI)injection on esophageal cancer cells and related mechanism.Methods Esophageal cancer cells ECA-109 and TE-10 were treated with different concentrations of HDI for 24 h and 48 h,and the cell viability was detected by CCK-8 assay.The effect of HDI on the clone formation of ECA-109 and TE-10 cells was examined by the clone plate formation test.ECA-109 and TE-10 cells were divided into different groups,and their apoptosis and the expression of P38,ERK and JNK proteins were detected.Results HDI inhibited the survival rate of ECA-109 and TE-10 cells in a time and dose-dependent manner.The IC_(50)of 24 h HDI was 66.56 ml/L and 56.52 ml/L for ECA-109 and TE cells,respectively.HDI inhibited the clone formation of ECA-109 and TE-10 cells in a dose-dependent manner.Compared with X-ray exposure,HDI+ray treatment remarkably increased the apoptosis of ECA-109 and TE-10 cells in a dose-dependent manner(P<0.05).Compared with those treated with X-ray or HDI alone,ECA-109 and TE-10 cells showed significantly decreased levels of ERK,JNK and P38 proteins.Conclusions HDI can effectively improve the radiosensitivity of esophageal cancer cells,which may be related to reducing the phosphorylation levels of ERK,JNK and P38 in the MAPK pathway.

关 键 词：白花蛇舌草注射液 食管鳞癌细胞 放疗 MAPK通路 

分 类 号：R285[医药卫生—中药学]