刺梨提取物抗炎活性及对溃疡性结肠炎小鼠的治疗作用  

Anti-inflammatory activity of Cili extracts and its therapeutic effect on ulcerative colitis model of mice

作　　者：王丽 李立郎[1,2] 李齐激 王瑜[1,2] 杨小生 WANG Li;LI Li-lang;LI Qi-ji;WANG Yu;YANG Xiao-sheng(State Key Laboratory of Functions and Applications of Medicinal Plants,Guiyang,Guizhou 550014,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang,Guizhou 550014,China)

机构地区：[1]省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《食品与机械》2023年第1期164-169,共6页Food and Machinery

基　　金：贵州省科技计划项目(编号:黔科合成果[2022]一般025);贵州省科技创新能力建设专项(编号:黔科合服企[2020]4013号);贵州省教育厅高等学校特色重点实验室建设项目(编号:黔教合KY字[2020]018号)。

摘　　要：目的:探讨刺梨提取物治疗肠炎腹泻的作用。方法:用不同质量浓度(1.25,2.50,5.00,10.00,20.00μg/mL)的刺梨提取物处理RAW264.7细胞,MTT法检测细胞活力;用不同浓度刺梨提取物处理RAW264.7细胞,再以脂多糖(LPS)诱导建立细胞炎症模型,利用Griess法检测细胞培养上清液中NO的含量。以3%葡聚糖硫酸钠(DSS)诱导建立小鼠溃疡性结肠炎(UC)模型,观察造模及药物治疗期间小鼠体征变化,评估疾病活动指数(DAI),测定小鼠结肠长度、结肠湿重指数及脾脏重量;HE染色观察小鼠结肠组织病理学形态;Western blot法及免疫组化法检测小鼠结肠组织中Keap1及Nrf2蛋白水平。结果:刺梨提取物质量浓度≤10μg/mL时对细胞活力无显著影响(P>0.05);刺梨提取物可抑制NO的释放,随着给药浓度的增加,抑制作用越明显(P<0.05或P<0.01)。与模型组比较,刺梨提取物各组小鼠的结肠长度较长,结肠湿重指数、脾脏重量及DAI分数降低(P<0.05或P<0.01);HE染色结果显示刺梨提取物对UC小鼠肠道黏膜层显示出了较好的保护作用;Western blot及免疫组化结果显示刺梨提取物能下调Keap1的表达水平,上调Nrf2的表达水平。结论:刺梨提取物具有较好的体外抗炎活性,对溃疡性结肠炎小鼠有治疗作用。Objective:This study aimed to investigate the effect of Cili extracts in the treatment of enteritis and diarrhea.Methods:The RAW 264.7 cells were treated with different concentrations(1.25,2.50,5.00,10.00,20.00μg/mL)of Cili extracts,and then the cell viability was determined by MTT method.RAW 264.7cells were treated with different concentrations of Cili extracts,and then lipopolysaccharide(LPS)was used to induce the cell inflammation model.The content of NO in cell culture supernatant was detected by Griess method.The UC mice model was induced by 3%DSS.The changes of signs during the modeling and drug treatment were observed,and the disease activity index(DAI)was evaluated.The colon length,colon wet weight index and spleen weight were measured.HE staining was used to observe the pathological morphology of colon.The protein expression of Keapl and Nrf2 in colon tissues were detected by western blot and immunohistochemistry.Results:The concentration of Cili extract≤10μg/mL had no significant effect on cell viability(P>0.05).Cili extract inhibited the release of NO,and the inhibitory effect was more obvious with the increase of drug concentration(P<0.05 or P<0.01).Compared with the model group,the colon length of Cili extract group was longer,and the colon wet weight index,spleen weight and DAI fraction were decreased(P<0.05 or P<0.01).HE staining showed that Cili extract had a better protective effect on the intestinal mucosa of UC mice.Western blot and immunohistochemistry showed that Cili extract down-regulated the expression level of Keapl and up-regulated the expression level of Nrf2.Conclusion:Cili extract had good anti-inflammatory activity in vitro and expressed therapeutic effect on UC mice.

关 键 词：刺梨提取物 抗炎活性 葡聚糖硫酸钠 溃疡性结肠炎 作用机制 

分 类 号：R285.5[医药卫生—中药学]