Identification of coexistence of biological and non-biological aerosol particles with DAPI (4′,6-diamidino-2-phenylindole) stain  

作　　者：Ting Liu Jiaquan Zhang Junji Cao Han Zheng Changlin Zhan Hongxia Liu Lili Zhang Kai Xiao Shan Liu Dong Xiang Daizhou Zhang 

机构地区：[1]School of Environmental Science and Engineering,Hubei Polytechnic University,Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation,Huangshi 435003,China [2]Institute of Atmospheric Physics,Chinese Academy of Sciences,Beijing 100029,China [3]School of Computer Science and Artificial Intelligence,Wuhan University of Technology,Wuhan 430063,China [4]Faculty of Environmental and Symbiotic Sciences,Prefectural University of Kumamoto,Kumamoto 862-8502,Japan

出　　处：《Particuology》2023年第1期49-57,共9页颗粒学报（英文版）

基　　金：supports from the Open Fund of Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation(grant No.2014104);the Research Project of Hubei Provincial Department of Education(grant No.D20184502);the National Natural Science Foundation of China(grant No.42107088).

摘　　要：The fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI) has been widely used to stain microorganisms in various environment media. We applied DAPI fluorescence enumeration to airborne microorganisms and found that non-biological particles, including organic compounds, minerals, and soot, were also visible upon exposure to UV excitation under fluorescence microscope. Using laboratory-prepared biological particles as the control, we investigated the feasibility of identifying both biological and non-biological particles in the same sample with DAPI staining. We prepared biological (bacterial, fungi, and plant detritus) and non-biological (biochar, soot, mineral, metal, fly ash, salt) particles in the laboratory and enumerated the particles and their mixture with DAPI. We found that mineral particles were transparent, and biochar, soot, metals and fly ash particles were black under a filter set at excitation 350/50 nm and emission 460/50 nm bandpass (DAPI-BP), while biological particles were blue, as expected. Particles of the water-soluble salts NaCl and (NH_(4))_(2)SO_(4) were yellow under a filter set at excitation 340–380 nm and emission 425 nm long pass (DAPI-LP). Case studies with samples of dustfall, atmospheric aerosols and surface soils could allow for the quantification of the relative number of different types of particles and particles with organic matter or salt coating as well. Fluorescence enumeration with DAPI stain is thus able to identify the co-existence of biological and non-biological particles in the air, at least to the extent of those examined in this study.

关 键 词：4' 6-diamidino-2-phenylindole(DAPI) Biological particles Nonbiological particles Atmospheric aerosols 

分 类 号：TG1[金属学及工艺—金属学]