高糖培养对大鼠肾小球系膜细胞内胆固醇含量的影响  

Effect of high glucose on intracellular cholesterol content in rat glomerular mesangial cells

作　　者：张国娟[1] 王宏娟[2] 徐世明[2] 梁巧静 Zhang Guojuan;Wang Hongjuan;Xu Shiming;Liang Qiaojing(Department of Nephrology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Biochemistry and Molecular Biology,Yanjing Medical College,Capital Medical University,Beijing 101300,China)

机构地区：[1]首都医科大学附属北京同仁医院肾内科,北京100730 [2]首都医科大学燕京医学院生化与分子生物学教研室,北京101300

出　　处：《中国医师杂志》2023年第2期242-246,251,共6页Journal of Chinese Physician

基　　金：首都医科大学基础-临床科研合作基金(17JL29)。

摘　　要：目的观察高糖培养对大鼠肾小球系膜细胞内胆固醇含量的影响并探讨其可能机制。方法体外培养大鼠肾小球系膜细胞,分为高糖培养组(高糖组,培养基糖浓度为30 mmol/L)及正常糖培养组(正常组,培养基糖浓度为5.5 mmol/L)。分别于培养24、36、48 h时,用油红O染色及分光光度计比色法测定细胞内脂质含量,采用细胞内蛋白印记法检测细胞低密度脂蛋白胆固醇受体(LDLR)和三磷酸腺苷结合盒A1(ABCA1)的表达。结果油红O染色提示培养36 h时高糖组细胞内脂滴少于正常组,培养24 h及48 h时两组无明显差别。高糖组大鼠肾小球系膜细胞在培养36 h时细胞内总胆固醇(TC)及胆固醇酯(CE)显著低于正常组(P=0.028、0.029),而游离胆固醇(FC)差异无统计学意义(P=0.306)。培养24、48 h时,两组间细胞内TC、CE及FC差异均无统计学意义(均P>0.05)。高糖组大鼠肾小球系膜细胞LDLR表达量在培养24、36、48 h时均显著低于正常组(P=0.043、0.004、0.028),ABCA1表达量在培养24、36、48 h时均显著高于正常组(P=0.050、0.009、0.006)。结论高糖培养可引起大鼠肾小球系膜细胞LDLR的表达下降、ABCA1的表达增多,从而引起细胞内脂质含量减少。Objective To investigate the effect of high glucose on intracellular cholesterol content in rat glomerular mesangial cells and the underlying mechanism.Methods Rat glomerular mesangial cells were cultured in vitro and divided into high glucose culture group(high glucose group,medium glucose concentration of 30 mmol/L)and normal glucose culture group(normal group,medium glucose concentration of 5.5 mmol/L).Lipid content was determined by oil red O staining and spectrophotometer colorimetry at 24,36 and 48 h of culture.Intracellular protein imprinting was used to detect the expression of low density lipoprotein cholesterol receptor(LDLR)and adenosine triphosphate binding cassette A1(ABCA1).Results Oil red O staining showed that the intracellular lipid drops in the high glucose group were less than those in the normal group at 36 h,and there was no significant difference between the two groups at 24 h and 48 h of culture.The total cholesterol(TC)and cholesterol ester(CE)in glomerular mesangial cells of rats in the high glucose group were significantly lower than those in the normal group(P=0.028,0.029),while there was no significant difference in the free cholesterol(FC)between the two groups(P=0.306).There was no significant difference in TC,CE and FC between the two groups at 24 and 48 h of culture(all P>0.05).The expression of LDLR in mesangial cells of rats in high glucose group was significantly lower than that in normal group at 24,36 and 48 h of culture(P=0.043,0.004,0.028),and the expression of ABCA1 was significantly higher than that in normal group at 24,36 and 48 h of culture,(P=0.050,0.009,0.006).Conclusions High glucose may reduce intracellular cholesterol content in rat glomerular mesangial cells by reducing LDLR protein expression and increasing ABCA1 protein expression.

关 键 词：肾小球系膜细胞 胆固醇 高糖培养 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]