离焦对表面等离激元共振显微成像的影响  

Effect of Defocus on Surface Plasmon Resonance Microscopy

作　　者：霍童 王靓安 王雪 孙晓娟 孙旭晴 刘虹遥[1] 黄尚永 余辉 路鑫超[1] 黄成军[1,3] Huo Tong;Wang Liang′an;Wang Xue;Sun Xiaojuan;Sun Xuqing;Liu Hongyao;Huang Shangyong;Yu Hui;Lu Xinchao;Huang Chengjun(Health Electronics Center,Institute of Microelectronics of Chinese Academy of Sciences,Beijing 100029,China;School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Science,Beijing University of Civil Engineering and Architecture,Beijing 102616,China)

机构地区：[1]中国科学院微电子研究所健康电子研发中心,北京100029 [2]上海交通大学生物医学工程学院,上海200240 [3]中国科学院大学,北京100049 [4]北京建筑大学理学院,北京102616

出　　处：《光学学报》2022年第23期174-179,共6页Acta Optica Sinica

基　　金：中国科学院科研装备研制项目(YJKYYQ20190056);2020年北京高等学校高水平人才交叉培养“实培计划”项目;北京市自然科学基金(4192063,4182073);广州市科技计划(201604020005);广东省科技计划(2016A040403086);清华大学环境模拟与污染控制国家重点联合实验室开放课题(18K07ESPCT)。

摘　　要：表面等离激元共振显微成像(SPRM)技术具有高灵敏度、快速实时等优点,已经被广泛应用于纳米检测、生物医学和环境监测等领域中。由于倏逝场的界面传输特性,故SPRM具有特殊的点扩散函数,可从其中提取出丰富的待测物信息。然而,离焦成像会影响成像特征,导致无法准确获取待测物信息。因此,定量研究离焦对SPRM的影响至关重要。通过仿真计算与实验,定量研究了离焦对SPRM的影响,并实现了单个聚苯乙烯颗粒的SPRM成像。所提方法可用于SPRM离焦状态的快速判断,并反推出准确的离焦偏移量,实现快速对焦,改善SPRM技术在长时程观测中的性能。Due to the high sensitivity and real-time detection,surface plasmon resonance microscopy(SPRM)has been widely used in nano-detection,biomedicine,and environmental monitoring.As the evanescence wave propagates along the interface,a special point spread function of SPRM is formed,from which rich information of the analyte can be retrieved.However,the defocus can affect the imaging pattern,which hinders the acquisition of accurate analyte information.As a result,the quantitative study on the effect of defocus on SPRM is crucial.This study quantitatively analyzes the effect of defocus on SPRM both theoretically and experimentally,and realizes the SPRM imaging of single polystyrene nanoparticles.The proposed method can be used to rapidly judge the defocus status of SPRM,retrieve the accurate defocus displacement to achieve fast re-focusing,and improve the SPRM performance in long-term observation.

关 键 词：表面光学 表面等离激元共振显微成像 单纳米颗粒 离焦 干涉条纹 定量分析 

分 类 号：O436.2[机械工程—光学工程]