抗体靶向性和高增强分辨率的多重散射无花果式纳米造影剂的构建及验证  

Construction and validation of a multiple scattering FIG nano contrast agent with antibody targeting and high enhancement resolution

作　　者：胡锦伟 田倚 樊浩 刘玉桃 朱钰璞 宋朝君 范黎 HU Jinwei;TIAN Yi;FAN Hao;LIU Yutao;ZHU Yupu;SONG Chaojun;FAN Li(Department of Pharmaceutical Analysis,Department of Pharmacy,Air Force Medical University,Xi'an 710032;Department of Medical Oncology,Air Force Medical Center of PLA,Beijing 100142;School of Life Science,Northwestern Polytechnical University,Xi'an 710072,China)

机构地区：[1]空军军医大学药学系药物化学与药物分析学教研室,陕西西安710032 [2]空军特色医学中心肿瘤科,北京100142 [3]西北工业大学生命学院,陕西西安710072

出　　处：《细胞与分子免疫学杂志》2022年第12期1130-1137,共8页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(82171962)。

摘　　要：目的在有限纳米尺寸内最大化增强超声造影剂成像对比度,同时,通过特异性靶向抗体修饰增强成像准确度。方法构建以磷脂纳米囊泡为外壳,沿内壁负载中空自解离二氧化硅纳米粒子,表面修饰葡萄糖转运蛋白1(GLUT-1)特异性抗体的多重散射“无花果”式纳米造影剂,并在体内外模型中探究该纳米造影剂成像准确度和对比度。结果体外脱气水指套模型成像结果显示,抗体靶向和非靶向的纳米造影剂成像效果相当,但二者的成像效果均优于声诺维(Sonovue);体内成像结果显示,抗体靶向的纳米造影剂成像效果优于非靶向造影剂,且二者均优于Sonovue。结论抗体靶向的多重散射“无花果”式纳米造影剂兼具高靶向性和高增强分辨率特性。Objective This study is aimed to maximize the contrast of ultrasound imaging in limited nanometer size range,and enhance the accuracy of imaging by introducing specific targeting antibody.Methods A multiple scattering“FIG”nano contrast agent system was established by conventional nanobubble synthesis method.The structure of“FIG”nano contrast agent was composed of phospholipid as the bubble shell with self-decomposable nanoparticles loaded on the inner wall,and the specific antibody to glucose transporter 1(GLUT-1)on the surface.Characterizations,in vitro and in vivo studies were employed to investigate the contrast and the accuracy of established nano bubble contrast agent.Results The in vitro imaging results revealed that with or without targeting ligand decoration,the“FIG”nano contrast agent system presented similar imaging enhancement,when compared with clinical used contrast imaging agent Sonovue.However,the imaging studies results in vivo showed that the“FIG”nano contrast agent system with targeting ligand decoration presented better imaging contrast and accuracy than free“FIG”nano contrast agent system,and both were better than Sonovue.ConclusionThe antibody-targeted multiple scattering“FIG”nano contrast agents possesses the characteristics of high targeting and high enhancement resolution.

关 键 词：抗体靶向 超声造影剂 纳米囊泡 自解离二氧化硅纳米粒子 

分 类 号：R392.12[医药卫生—免疫学] R445.1[医药卫生—基础医学] R730.41[生物学—生物工程] Q81R914.3