3D-printed Mechanically Strong Calcium Phosphate Cement Scaffold with Metformin/Stem Cell-encapsulating Alginate Microbeads for Bone Tissue Engineering  

作　　者：Xiong Xiong Yuanyuan Chen Rupan Yuan Gengtao Qiu Michael D.Weir Hockin H.K.Xu Jin Liu Jianping Ruan Xiaofeng Chang Shuxin Qu 

机构地区：[1]School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,610031,China [2]Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs,School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,610031,China [3]Key Lab of Advanced Technologies of Materials,Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu,610031,China [4]Department of Advanced Oral Sciences and Therapeutics,University of Maryland School of Dentistry,Baltimore,MD,21201,USA [5]Center for Stem Cell Biology and Regenerative Medicine,University of Maryland School of Medicine,Baltimore,MD,21201,USA [6]University of Maryland Marlene and Stewart Greenebaum Cancer Center,University of Maryland School of Medicine,Baltimore,MD,21201,USA [7]Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research,Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases,College of Stomatology,Xi’an Jiaotong University,Xi’an,Shannxi,710004,China

出　　处：《Journal of Bionic Engineering》2022年第6期1658-1670,共13页仿生工程学报（英文版）

基　　金：National Natural Science Foundation of China(Grant No.52035012);Fundamental Research Funds for the Central Universities(2682020ZT91);Basic Research Foundation Key Project of Sichuan Province(2021JY0046);Basic Research Foundation of Sichuan Province(2022JDRC0088).

摘　　要：The utilization of Calcium Phosphate Cement(CPC)is limited due to its low mechanical strength and difficulty to seed cells deep into the scaffold.The objectives of this study were to:(1)develop a 3D-printed CPC-dopamine-metformin scaffold encapsulating human periodontal ligament stem cells(hPDLSCs),(2)investigate the effect of dopamine on the performance of CPC,and(3)evaluate the effect of microbead degradation and metformin release on the osteogenic differentiation of the released hPDLSCs.The mechanical property of the CPC scaffolds was elevated by adding dopamine,and the CPC scaffold with 7 wt.%dopamine had the highest compressive strength(7.35 MPa).Four types of microbeads with different content of alginate(oxidized alginate),hPDLSCs,and 2%metformin were fabricated.Morphological and cell counting kit tests confirm that the hPDLSCs are protected by microbeads encapsulation during the CPC setting process.The alkaline phosphatase test indicates that the osteogenic differentiation of hPDLSCs was enhanced by the fast release of cells and metformin.The microbeads consisting of 2%oxidized alginate and 2%metformin were optimal for cell delivery due to favorable cell release and osteogenic differentiation.This CPC scaffold is promising used for bone regeneration in dental,craniofacial,and orthopedic applications.

关 键 词：Calcium phosphate cement 3D Printing BIONIC Alginate microbeads Metformin 

分 类 号：R318.08[医药卫生—生物医学工程]