镉胁迫下转BADH基因番茄光系统II特性和光保护响应  

Photosystem II characteristics and photoprotection response of BADH-transgenic tomato plants under cadmium stress

作　　者：卫丹丹 张会玲 王丙全 苗筠杰 徐琦 杨兴洪[2] WEI Dandan;ZHANG Huiling;WANG Bingquan;MIAO Junjie;XU Qi;YANG Xinghong(Xinzhou Teachers University,Xinzhou,Shanxi 034000,China;State Key Laboratory of Crop Biology,Shandong Agricultural University,Taian,Shandong 271018,China;Agricutural and Rural Bureau of Zhangqiu District,Jinan City,Jinan 250200,China;Maize Research Institute of Shanxi Agricultural University,Xinzhou,Shanxi 034000,China)

机构地区：[1]忻州师范学院,山西忻州034000 [2]山东农业大学作物生物学国家重点实验室,山东泰安271018 [3]济南市章丘区农业农村局,济南250200 [4]山西农业大学玉米研究所,山西忻州034000

出　　处：《植物生理学报》2022年第12期2295-2304,共10页Plant Physiology Journal

基　　金：山东农业大学国家重点实验室开放课题(2017KF10);山西省基础研究计划(自由探索类)青年科学研究项目(20210302123366);忻州师范学院科研基金(2021KY12)。

摘　　要：以野生型(wild-type,WT)和转BADH基因番茄为材料,研究镉(cadmium,Cd)胁迫下叶片叶绿体结构和光系统Ⅱ(PSⅡ)特性变化,同时探究D1蛋白周转和叶黄素循环在转BADH番茄光保护机制中的作用。结果表明:高浓度Cd^(2+)(>0.5 mmol·L^(-1))抑制植株生长,各番茄株系叶片的性能指数(PI)下降。JIP-测定结果显示,Cd处理后转BADH株系单位面积热耗散(DI_(o)/CS_(m))和Q_(A)还原次数(N)增大。Cd胁迫下,二硫苏糖醇(1,4-dithiothreitol,DTT)和硫酸链霉素(streptomycin,SM)处理均使离体叶片PI、单位面积电子传递的量子产额(ET_(o)/CS_(m))等荧光参数发生变化,其中DTT处理的各参数变化幅度较大。Cd胁迫下转BADH番茄株系具有更完善的光保护机制,叶黄素循环在转BADH番茄叶片光保护机制中具有重要作用,且作用大于D1蛋白周转。Wild-type(WT) and BADH-transgenic tomato lines were used to study the changes of chloroplast structure and photosystem Ⅱ(PSⅡ) characteristics of leaves under cadmium (Cd) stress.We also studied the role of D1 protein turnover and xanthophyll cycle in photoprotection mechanism of BADH-transgenic tomato plants.The results showed that high concentration of Cd^(2+)(>0.5 mmol·L^(-1)) inhibited the growth of plants.Performance index(PI) of all tomato leaves were decreased.JIP-test revealed that dissipated energy flux per cross section (DI_(o)/CS_(m)) and the number of Q_(A)reduction events (N) were increased in BADH-transgenic lines after Cd treatment.Under Cd stress,fluorescence parameters such as PI and electron transport flux per cross section (ET_(o)/CS_(m)) were changed after DTT and SM treatment in isolated leaves.The parameters of leaves treated with DTT had changed even more.There was a better photoprotection mechanism in BADH-transgenic tomato seedlings under Cd stress.Xanthophyll cycle plays an important role in the photoprotection mechanism of BADH-transgenic tomato leaves,and its role is greater than D1 protein turnover.

关 键 词：镉污染 番茄 甜菜碱 光系统Ⅱ 叶黄素循环 

分 类 号：X53[环境科学与工程—环境工程] X173[农业科学—蔬菜学] S641.2[农业科学—园艺学]