蜗牛黏液糖胺聚糖的化学结构及抗凝活性  

Chemical structure of glycosaminoglycan from the snail Achatina fulica mucus and its anticoagulant activity

作　　者：高冬秀 周立晓 邓拓 付欣蕊 吴月波 杨莲[2] 罗兰 胡琳 吴明一[2] GAO Dong-xiu;ZHOU Li-xiao;DENG Tuo;FU Xin-rui;WU Yue-bo;YANG Lian;LUO Lan;HU Lin;WU Ming-yi(School of Ethnic Medicine,Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education,Yunnan Minzu University,Kunming 650500,China;Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China)

机构地区：[1]云南民族大学民族医药学院民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]中国科学院昆明植物研究所,云南昆明650204

出　　处：《云南民族大学学报（自然科学版）》2023年第1期6-12,共7页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：中国科学院青年创新促进会优秀会员资助项目(Y2021104).

摘　　要：研究了白玉蜗牛黏液中糖胺聚糖的化学结构及其对血液凝固的影响.采用酶解法提取白玉蜗牛黏液粗多糖,季铵盐沉淀法和离子交换柱层析法纯化获得多糖SMG-2.单糖组成分析以及紫外、红外、核磁共振等波谱方法对SMG-2的化学组成与结构进行分析研究,体外抗凝血实验分析SMG-2对人质控血浆凝血时间的影响.结果表明,SMG-2由L-艾杜糖醛酸和D-乙酰氨基葡萄糖组成的类肝素糖胺聚糖,因其肝素抗凝五糖序列而无显著抗凝血活性.The chemical structure of glycosaminoglycan from the snail Achatina fulica mucus and its anticoagulation were investigated.The crude polysaccharide SMG-1 were extracted from the snail mucus by enzymatic hydrolysis,and the purified polysaccharide SMG-2 was obtained by quaternary ammonium salt precipitation and ion exchange column chromatography.The chemical structure of SMG-2 was clarified by monosaccharide composition analysis and UV,IR and NMR spectroscopy.The anticoagulant activity of SMG-2 was evaluated by in vitro using human quality control plasma.The results showed that SMG-2 was a heparin-like glycosaminoglycan which was composed of iduronic acid and N-acetyl-glucosamine,and had no significant anticoagulant activity due to lacking of heparin-pentosaccharide sequence.

关 键 词：白玉蜗牛 黏液 多糖 化学结构 抗凝活性 

分 类 号：R932[医药卫生—生药学]