核壳型磁性分子印迹聚合物制备--以马尿酸为模板  

Preparation for Core-shell Magnetic Molecularly Imprinted Polymers Using Hippuric Acid Template

作　　者：樊世萌 刘恋 欧阳敬禹 刘浩 尤祥宇 李玲玲 苏江涛 FAN Shimeng;LIU Lian;OUYANG Jingyu;LIU Hao;YOU Xiangyu;LI Lingling;SU Jiangtao(School of Biological Engineering and Food,Hubei Univ.of Tech.,Wuhan 430068,China;Medical School,Wuhan Univ.of Arts and Science,Wuhan 430345,China)

机构地区：[1]湖北工业大学生物工程与食品学院,湖北武汉430068 [2]武汉文理学院医学院,湖北武汉430345

出　　处：《湖北工业大学学报》2023年第1期53-58,共6页Journal of Hubei University of Technology

基　　金：国家自然科学基金青年科学基金(81703409);湖北省教育厅科学研究计划(B2017047,B2018421)。

摘　　要：用表面分子印迹技术,以氨基化磁性纳米颗粒为核,以马尿酸(HA)为模板分子、甲基丙烯酸(MAA)为功能单体、偶氮二异丁腈(AIBN)为引发剂、乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,制备核壳型马尿酸磁性分子印迹聚合物(HA-MMIPs)。通过电镜对其结构进行表征,并通过动力学吸附、等温吸附、比较HA-MMIPs与人血清白蛋白(HSA)对HA的吸附等实验,对其性能进行评价。结果表明,HA-MMIPs对HA具有较好的吸附量和印迹效率,在吸附HA上表现出对HSA的明显优势。HA五轮吸附洗脱的循环实验表明,HA-MMIPs具有很好的重复再利用能力。这项研究证明HA-MMIPs对马尿酸分子具有良好的分离与吸附能力,对于尿毒症患者体内马尿酸分子的分离与吸附可能存在潜在的优势。Using surface molecular imprinting technology,aminated magnetic nanoparticles as the core,hippuric acid(HA)as the template molecule,methacrylic acid(MAA)as the functional monomer,azobisisobutyronitrile(AIBN)as the initiator,and ethyl acetate Glycol dimethacrylate(EGDMA)was used as a crosslinking agent to prepare core-shell hippuric acid magnetic molecularly imprinted polymers(HA-MMIPs).Its structure was characterized by electron microscopy,and its performance was evaluated by experiments such as kinetic adsorption,isothermal adsorption,and comparison of HA-MMIPs and HSA's adsorption of HA.The results show that HA-MMIPs have better adsorption capacity for HA,better imprinting efficiency,and show obvious advantages over HSA in adsorbing HA;through at least five rounds of adsorption-elution cycle experiments on HA,it is shown that HA-MMIPs have a good ability to reuse.This study proves that HA-MMIPs have good separation and adsorption ability to hippuric acid,and may have potential advantages for the separation and adsorption of hippuric acid in uremia patients.

关 键 词：马尿酸 尿毒症毒素 分子印迹 人血清白蛋白 

分 类 号：TB383.1[一般工业技术—材料科学与工程]