基于空间频域成像的烧伤程度无创定量评估  

Noninvasive Quantitative Assessment of Burn Degree Based on Spatial Frequency-Domain Imaging

作　　者：钟晓雪 黄国武 缪弘波 胡城豪 刘威 孙春容 陈志华 李港宁 曹自立[1] 金鑫 林维豪[1] Zhong Xiaoxue;Huang Guowu;Miu Hongbo;Hu Chenghao;Liu Wei;Sun Chunrong;Chen Zhihua;Li Gangning;Cao Zili;Jin Xin;Lin Weihao(College of Optometry(College of Biomedical Engineering)Wenzhou Medical University,Wenzhou 325035,Zhejiang,China)

机构地区：[1]温州医科大学眼视光学院(生物医学工程学院),浙江温州325035

出　　处：《中国激光》2022年第24期108-117,共10页Chinese Journal of Lasers

基　　金：国家自然科学基金(61905181);浙江省基础公益研究计划项目(LQ18F050005);温州市科技计划项目(Y20170219)。

摘　　要：针对目前临床上尚缺乏有效可行的烧伤程度精准诊断的难题,本课题组研究了基于空间频域成像的烧伤程度无创定量评估方法。本课题组通过基于单次快照多频解调方法的空间频域成像技术,实时、大面积、高分辨地反演出了与烧伤组织结构、生理特性紧密相关的光学参数(吸收系数与约化散射系数),并结合系统聚类方法与多参数降维分析,提高了烧伤程度分类的准确性,缩短了分类时间。鼠烧伤模型的实验结果表明:动态监测光学参数的变化趋势可以显著区分出三种不同的烧伤程度,系统聚类分析缩短了分类时间,基于主因子的多参数降维分析表现出了更强的抗干扰性。本文方法为临床烧伤的早期诊断提供了一种极具潜力的实现途径。Objective The increasing burn mortality rate places an urgent need for accurate diagnosis and treatment of burns.C urrently,the third-degree quartile is internationally used to classify the degree of burns based on burn depth,and clinical treatment methods for different degrees of burns are significantly dissimilar.Burn surgeons overestimating the severity of burns can lead to unnecessary surgery,whereas underestimating them leads to treatment delays and worsening of the burn conditions.In addition,studies have shown that burn severity changes dynamically over time,with superficialⅡburn w orsening to deepⅡorⅢburns within 48 h of burn occurrence.Therefore,overcoming the defects of subjective jud gment using the naked eye and quantitatively monitoring the dynamic changes in the burn degree in real time has become a challenge in the early diagnosis of burns.Burn diagnosis methods based on photonics,such as near-infrared spectroscopy,reflective confocal microscopy,and laser Doppler flowmetry,are developing rapidly.However,their clinical application is limited owing to low accuracy,invasiveness,high detection environment requirements,and high costs.In this study,a noninvasive quantitative method for assessing the burn degree was developed based on spatial frequency-domain imaging(SFDI).Combined with the systematic clustering method and multiparameter dimensionality reduction analysis,the proposed method results in improved classification accuracy of different burn degrees and shortened classification time,thus indicating the potential for early diagnosis of clinical burns.Methods In this study,the SFDI technique was applied to a rat burn model.First,the backs of Sprague-Dawley(SD)r ats were depilated,and a thermostatic iron heated to 100℃was used on the backs of the anesthetized SD rats for 4,12,and 24 s,respectively,to establish a rat burn model with different burn degrees.Next,the sinusoidally modulated structural patterns were projected onto the surface of each burned area,and the backscattered structural p

关 键 词：医用光学 医学生物成像 空间频域成像 烧伤程度评估 组织光学参数 

分 类 号：O436[机械工程—光学工程]