PRC1在胰腺癌中的细胞生物学功能及临床意义  

The cell biological function and clinical significance of PRC1 in pancreatic carcinoma

作　　者：马丹丹 张翌 林振宇 董庆泰 萧正康 李中虎 张智勇 金炜东 Ma Dandan;Zhang Yi;Lin Zhenyu;Dong Qingtai;Xiao Zhengkang;Li Zhonghu;Zhang Zhiyong;Jin Weidong(Dept of General Surgery,General Hospital of Central Theater Command,Wuhan 430070)

机构地区：[1]中部战区总医院普通外科,武汉430070

出　　处：《安徽医科大学学报》2023年第2期189-195,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81902501);湖北省自然科学基金(编号:2021CFB500)。

摘　　要：目的探讨细胞质分裂调控蛋白1(PRC1)在胰腺癌组织中的表达、预后及其对胰腺癌细胞系SW1990细胞生物学功能的影响及其相关机制。方法采用GEPIA数据库分析PRC1在胰腺癌及正常胰腺组织中的表达差异。采用脂质体3000转染质粒或siRNA方法实现对PRC1的过表达及干扰,分别采用CCK-8细胞增殖实验、Transwell细胞侵袭实验、流式细胞术检测PRC1对SW1990细胞增殖能力、侵袭能力及凋亡率的影响。从TCGA获取胰腺癌临床病例资料,统计分析PRC1表达量与胰腺癌患者临床病理特征的关系。STRING数据库分析与PRC1相互作用的蛋白网络。基因集富集分析预测PRC1在胰腺癌中调控的可能信号通路。结果GEPIA数据库分析结果显示PRC1在胰腺癌组织中的mRNA表达量显著高于正常胰腺组织(P<0.05)。CCK-8细胞增殖实验、Transwell细胞侵袭实验、流式细胞术结果表明,过表达PRC1促进胰腺癌SW1990细胞增殖、侵袭并抑制其凋亡(P<0.01),而沉默PRC1则抑制SW1990细胞增殖、侵袭并诱导其凋亡(P<0.01)。TCGA数据库分析结果显示PRC1 mRNA表达水平和M分期是胰腺癌患者预后的独立危险因素(P<0.05)。STRING数据库结果表明:PRC1与PLK1等蛋白存在相互作用。GSEA研究结果显示:PRC1 mRNA高表达样本富集到P53信号通路等相关基因集(P<0.05)。结论PRC1在胰腺癌中高表达且与胰腺癌的增殖、凋亡、侵袭及预后相关,PRC1可能通过调节PLK1等相互作用蛋白及调控P53等信号通路在胰腺癌中发挥功能。Objective To investigate the expression and prognosis of protein regulator of cytokinesis 1(PRC1)in pancreatic carcinoma tissues.Moreover,to explore the effects of PRC1 on the biological functions of pancreatic carcinoma cell line SW1990 and its related mechanisms.Methods The GEPIA database was used to analyze the expression difference of PRC1 in pancreatic carcinoma tissues and normal pancreatic tissues.Overexpression and interference of PRC1 were achieved by Lipofectamine 3000 transfection plasmid or shRNA method.Then CCK-8 assay,Transwell assay and flow cytometry were used to detect the proliferation level,invasion ability and apoptosis of the SW1990 cells,respectively.The pancreatic carcinoma data were collected from the Cancer Genome Atlas(TCGA)database.The correlation between expression level of PRC1 and clinicopathological features of pancreatic carcinoma was analyzed.The STRING database was used to analyze the network of proteins interacting with PRC1.Gene set enrichment analysis(GSEA)was used to predict the possible signal pathways of PRC1 in pancreatic carcinoma.Results GEPIA database results showed that PRC1 expression in pancreatic carcinoma tissue was higher than that in normal pancreatic tissue(P<0.05).The results of CCK-8 assay,Transwell assay and flow cytometry showed that PRC1 overexpression significantly enhanced SW1990 cell proliferation,invasion and inhibited apoptosis(P<0.01).Whereas PRC1 interference significantly inhibited SW1990 cell proliferation,invasion and enhanced apoptosis(P<0.01).TCGA database data analysis identified PRC1 mRNA expression level and M stage were independent risk factors affecting the prognosis of pancreatic carcinoma(P<0.05).STRING database showed that there was an interaction between PRC1 and PLK1 and so on.GSEA research results showed that the PRC1 mRNA high expression samples were enriched into P53 signaling pathway and so on(P<0.05).Conclusion PRC1 is highly expressed in pancreatic carcinoma,and it is associated with proliferation,invasion,apoptosis and prognosis

关 键 词：胞质分裂调控蛋白1 胰腺癌 增殖 凋亡 侵袭 临床意义 

分 类 号：R657.5[医药卫生—外科学]