腹腔注射同种异体粪便滤液建立大鼠脓毒症模型  

Establishment of the rat sepsis model by intraperitoneal injection of allogenic fecal filtrate

作　　者：刘辉 刘军超[2] 王伟宝[3] 杜会博 李福龙[2] 牛春雨[4,5] 赵自刚 Liu Hui;Liu Junchao;Wang Weibao;Du Huibo;Li Fulong;Niu Chunyu;Zhao Zigang(Institute of Microcirculation/College of Basic Medicine,Hebei North University,Zhangjiakou 075000;Dept of Anesthesiology,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000;Dept of Clinical Laboratory,The Fourth Hospital of Zhangjiakou City,Zhangjiakou 075000;College of Basic Medicine,Hebei Medical University,Shijiazhuang 050017;Laboratory of Pathogenesis and Intervention of Acute Critical Illness of Hebei Province,Zhangjiakou 075000)

机构地区：[1]河北北方学院微循环研究所/基础医学院,张家口075000 [2]河北北方学院附属第一医院麻醉科,张家口075000 [3]张家口市第四医院检验科,张家口075000 [4]河北医科大学基础医学院,石家庄050017 [5]河北省急危重症发病机制及干预重点实验室,张家口075000

出　　处：《安徽医科大学学报》2023年第2期236-242,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81670446)。

摘　　要：目的探讨应用腹腔注射同种异体粪便滤液建立中重度脓毒症大鼠模型的方法。方法选择健康Wistar大鼠作为研究对象,明确同种异体粪便滤液的制备方法,腹腔注射不同浓度(0.5、1、2 g/kg)的同种异体粪便滤液,观察大鼠一般情况、生存率与脓毒症程度;确定最佳剂量后,观察模型成功率、血清炎性因子、D-乳酸(D-LA)、血清肠型脂肪酸结合蛋白(I-FABP)、活体肺功能以及肺、肝、肾组织损伤等指标。结果腹腔注射同种异体粪便滤液24 h后,1 g/kg组大鼠出现发热、呼吸急促、低血压,24 h生存率83.3%,48 h生存率16.7%,2 g/kg组大鼠24 h内全部死亡,确定1 g/kg剂量用于后续实验。注射粪便滤液24 h后,模型成功率为77.8%;大鼠血清白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、D-LA、I-FABP水平明显增高;肺组织出现严重水肿和出血等表现,肺功能有功能残气量、动态肺顺应性、前100毫秒用力呼气容积、呼气峰流量下降以及气道阻力、深吸气量增高等呼吸功能不全表现;肝、肾组织也出现不同程度的细胞水肿和炎性细胞浸润,血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、尿素氮(BUN)、肌酐(Cr)水平明显增高。结论腹腔注射同种异体粪便滤液(1 g/kg)可造成比较典型的大鼠脓毒症模型。Objective To explore the method of intraperitoneal injection of allogenic fecal filtrate to establish the rat model of moderate and severe sepsis.Methods The preparation method of allogeneic fecal filtrate was determined.Allogeneic fecal filtrate of different concentrations(0.5,1,2 g/kg)was injected intraperitoneally to observe the general situation,survival time and severe degree of sepsis of rats.After determining the optimal concentration,the success rate of the model,serum inflammatory factors,serum concentration of D-lactic acid(D-LA)and serum intestinal fatty acid binding protein(I-FABP),lung function changes,lung,liver and kidney tissue injury were further observed.Results After intraperitoneal injection of allogenic fecal filtrate for 24 h,the rats of 1 g/kg group presented fever,tachypnea and hypotension,the survival rate was 83.3% at 24 h and 16.7% at 48 h,2 g/kg group rats all died within 24 h,the dose of 1 g/kg was determined for subsequent experiments.Injected fecal filtrate for 24 h,the success rate of the sepsis model was 77.8%.The levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),D-LA and I-FABP in serum significantly increased.There were severe edema and bleeding in lung tissue,Pulmonary function appeared respiratory dysfunction,included functional residual capacity(FRC),quasi static compliance(Cdyn),forced expiratory volume for the first 100 milliseconds(FEV100),peak expiratory flow(PEF)decreased,airway resistance(RI),inspiratory capacity(IC)increased.Liver and kidney tissues also showed varying degrees of edema and inflammatory cell infiltration,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN)and creatinine(Cr)in serum significantly increased.Conclusion Intraperitoneal injection of allogenic fecal filtrate(1 g/kg)can produce a relative typical septic model in rats.

关 键 词：脓毒症 腹腔注射 粪便滤液 动物模型 

分 类 号：R459.7[医药卫生—急诊医学] R332[医药卫生—治疗学]