葛根素降低细胞内Ca^(2+)浓度并上调BDNF保护血管性痴呆大鼠海马神经细胞  

Puerarin reduces intracellular Ca^(2+)concentration and upregulates BDNF to protect hippocampal neurons in vascular dementia rats

作　　者：任宏伟[1] 陈明[1] 王虎清[1] 高震[1] 吴海琴[1] REN Hongwei;CHEN Ming;WANG Huqing;GAO Zhen;WU Haiqin(Department of Neurology,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院神经内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2023年第2期202-207,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助面上项目(No.81270415);陕西省重点研发计划项目(No.2017SF-181)。

摘　　要：目的观察葛根素对血管性痴呆(VD)大鼠海马神经细胞内Ca^(2+)浓度及脑源性神经生长因子(BDNF)的影响,探讨葛根素保护神经细胞的可能机制。方法雄性SD大鼠随机分为假手术组、模型组和葛根素干预组,采用间隔3 d结扎双侧颈总动脉法建立VD模型;术后2周用Morris水迷宫评估大鼠学习记忆能力,免疫组化和Western blotting检测大鼠海马组织BDNF的表达情况,流式细胞仪检测平均荧光强度来表示细胞内游离Ca^(2+)浓度。结果葛根素干预组Morris水迷宫逃避潜伏期显著缩短,海马BDNF表达量显著增加,海马神经细胞内Ca^(2+)浓度减低;与模型组相比,差异有统计学意义(P<0.05)。结论葛根素对VD大鼠具有神经保护作用,其作用机制可能与葛根素降低海马神经细胞内Ca^(2+)浓度及上调BDNF的表达有关。Objective To observe the effect of puerarin on the concentration of Ca^(2+)and the expression of brain derived neurotrophic factor(BDNF)in hippocampal neurons of vascular dementia(VD)rats so as to explore the mechanism of puerarin in protecting nerve cells.Methods Male SD rats were randomly divided into sham operation group,model group,and puerarin intervention group.The vascular dementia model was established by ligating bilateral common carotid arteries at intervals of 3 days.Two weeks after the operation,the learning and memory abilities of the rats were evaluated by Morris water maze,and the expression of BDNF in the hippocampus of the rats was detected by immunohistochemistry and Western blotting.The mean fluorescence intensity was measured by flow cytometry to represent the intracellular free Ca^(2+)concentration.Results In the puerarin intervention group,the rats’escape latency in Morris water maze was significantly shortened,the expression of BDNF in the hippocampus was significantly increased,and the concentration of Ca^(2+)in hippocampal neurons was decreased.Compared with the model group,the difference was statistically significant(all P<0.05).Conclusion Puerarin has neuroprotective effect on VD rats,and its mechanism may be related to the decrease of Ca^(2+)concentration in hippocampal neurons and the up-regulation of BDNF expression.

关 键 词：葛根素 血管性痴呆 钙离子浓度 脑源性神经生长因子(BDNF) 神经保护 

分 类 号：R285.5[医药卫生—中药学]