Advances in plastid transformation for metabolic engineering in higher plants  

作　　者：Sheng Yang Yi Deng Shengchun Li 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan,430062,China

出　　处：《aBIOTECH》2022年第3期224-232,共9页生物技术通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32071477);Key Research and Development Program of Hubei Province(2021BBA224);Innovation Base for Introducing Talents of Discipline of Hubei Province(2019BJH021,2021EJD025).

摘　　要：The plastid(chloroplast)genome of higher plants is an appealing target for metabolic engineering via genetic transformation.Although the bacterial-type plastid genome is small compared with the nuclear genome,it can accommodate large quantities of foreign genes that precisely integrate through homologous recombination.Engineering complex metabolic pathways in plants often requires simultaneous and concerted expression of multiple transgenes,the possibility of stacking several transgenes in synthetic operons makes the transplastomic approach amazing.The potential for extraordinarily high-level transgene expression,absence of epigenetic gene silencing and transgene containment due to the exclusion of plastids from pollen transmission in most angiosperm species further add to the attractiveness of plastid transformation technology.This minireview describes recent advances in expanding the toolboxes for plastid genome engineering,and highlights selected high-value metabolites produced using transplastomic plants,including artemisinin,astaxanthin and paclitaxel.

关 键 词：Plastid transformation Metabolic engineering Multiple transgenes High-level expression 

分 类 号：Q94[生物学—植物学]