FSH与GDF9对绵羊卵泡颗粒细胞中C型钠肽及其受体表达的影响  

Effects of FSH and GDF9 on the expression of NPPC and NPR2 in follicular granulosa cells of sheep

作　　者：王俊兰 赵勇超 王兆琛 张楠 张家新[1] WANG Junlan;ZHAO Yongchao;WANG Zhaochen;ZHANG Nan;ZHANG Jiaxin(Inner Mongolia Key Laboratory of Animal Genetics,Breeding and Reproduction/College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《黑龙江动物繁殖》2023年第1期1-7,共7页Heilongjiang journal of animal reproduction

基　　金：内蒙古自治区科技重大专项“内蒙古绒山羊种质资源保护、创新及智慧养殖技术集成示范”(2021ZD0012)。

摘　　要：为了阐明绵羊卵泡颗粒细胞功能调控机制,试验选取不同条件培养的绵羊卵泡壁层颗粒细胞(MGCs)和卵丘颗粒细胞(CCs),分别设置对照组、FSH组和GDF9+FSH组,利用qPCR、Western blot和免疫荧光法分别检测MGCs和CCs中NPPC和NPR2的mRNA和蛋白表达量。结果表明:对于壁层颗粒细胞,FSH组NPPC 12 h mRNA及12,24 h蛋白水平显著高于对照组(P<0.05),极显著低于GDF9+FSH组(P<0.01);体外培养16 h后,与对照组相比,FSH组NPR2 mRNA及蛋白表达水平显著提高(P<0.05),但与GDF9+FSH组差异不显著(P>0.05)。对于卵丘颗粒细胞,FSH组NPPC mRNA及蛋白水平极显著高于对照组和GDF9+FSH组(P<0.01);FSH组NPR2 mRNA及蛋白表达水平显著高于对照组和GDF9+FSH组(P<0.05)。说明FSH可以显著提高体外培养的绵羊卵泡颗粒细胞中NPPC、NPR2的表达;GDF9可以促进FSH诱导的壁层颗粒细胞中NPPC的表达,但抑制了FSH诱导的卵丘细胞颗粒细胞中NPR2的表达。In order to provide a basis for clarifying the functional regulation mechanism of sheep follicular granulosa cells,sheep mural granulosa cells(MGCs)and cumulus cells(CCs)cultured under different conditions were selected,and control group,FSH group and GDF9+FSH group were set respectively.The mRNA and protein expressions of NPPC and NPR2 in MGCs and CCs were detected by qPCR,Western blot and immunofluorescence,respectively.The results showed that the 12 h mRNA and 12,24 h protein levels of NPPC in MGCs in FSH group were significantly higher than those in control group(P<0.05),extremly lower than those in GDF9+FSH group(P<0.01).After 16 h in vitro culture,FSH significantly increased the mRNA and protein expression levels of NPR2 in MGCs compared with the control group(P<0.05),but the differences were not significant compared with GDF9+FSH group(P>0.05).The mRNA and protein levels of NPPC in CCs of FSH group were extremly higher than those in control group and GDF9+FSH group(P<0.01).The mRNA and protein expression levels of NPR2 in FSH group were significantly higher than those in control group and GDF9+FSH group(P<0.05).These results indicated that FSH could significantly increase the expressions of NPPC and NPR2 in sheep follicular granulosa cells cultured in vitro.GDF9 promoted the expression of NPPC in FSH-induced parietal MGCs,but inhibited the expression of NPR2 in FSH-induced CCs.

关 键 词：绵羊 颗粒细胞 NPPC NPR2 FSH GDF9 

分 类 号：S826.2[农业科学—畜牧学] Q786[农业科学—畜牧兽医]