钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制  

Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice

作　　者：魏倩倩 徐青山 潘林 孔亚丽 朱练峰 曹小闯 田文昊 刘佳[3] 金千瑜 项兴佳 张均华 朱春权 WEI Qianqian;XU Qingshan;PAN Lin;KONG Yali;ZHU Lianfeng;CAO Xiaochuang;TIAN Wenhao;LIU Jia;JIN Qianyu;XIANG Xingjia;ZHANG Junhua;ZHU Chunquan(School of Resources and Environmental Engineering,Anhui University,Hefei 230601,China;State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [3]江西省农业科学院土壤肥料与资源环境研究所,南昌330200

出　　处：《中国水稻科学》2023年第2期142-152,共11页Chinese Journal of Rice Science

基　　金：国家自然科学基金青年项目(31901452,42207415,32201702);国家自然科学基金面上项目(31872857,31771733);国家重点研发计划课题(2022YFD1500403,2022YFD2301404-2);水稻生物学国家重点实验室开放课题资助项目(20190402)。

摘　　要：【目的】阐明钙离子与硫化氢相互作用缓解水稻铝毒害的分子和生理机制。【方法】以Kasalath为试验材料,选取0μmol/L和30μmol/L AlCl_(3),0.1 mmol/L和0.5 mmol/L CaCl_(2),0.2μmol/L NaHS和100μmol/L硫化氢清除剂亚牛磺酸(HP)作为处理浓度,将种子置于30℃培养箱中黑暗培养24 h后取水稻根系,通过测定水稻根系伸长量、总铝含量、细胞汁液中铝含量、质外体中铝含量、细胞壁中铝含量、果胶含量、果胶甲酯酶活性以及OsSTAR2、Os NRAT1和Os FRDL4相对表达量,探究钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制。【结果】铝胁迫下,相较于0.1 mmol/L CaCl_(2)处理,0.5 mmol/L CaCl_(2)处理显著提高了水稻根系伸长量、硫化氢含量、总钙含量和细胞质中钙含量,显著降低了水稻根系的总铝含量,细胞液、质外体和细胞壁中的铝含量。铝胁迫下,硫氢化钠预处理后,水稻根系的伸长量在两种钙浓度下均显著增加,水稻的根尖铝含量、根系总铝含量、细胞液中铝含量、质外体中铝含量和细胞壁中铝含量在两种钙浓度下均显著降低,OsSTAR2和Os FRDL4相对表达量在两种钙浓度下均显著提高,Os NRAT1相对表达量在两种钙浓度下均显著降低。铝胁迫下,添加HP则呈现相反的结果。【结论】铝胁迫下,钙离子通过增加水稻根系硫化氢的生成,降低水稻根系对铝的吸收和积累,最终缓解铝对水稻根系伸长的抑制作用。【Objective】The objective of this study is to elucidate the molecular and physiological mechanisms of the interaction between calcium ion(Ca^(2+))and hydrogen sulfide(H2S)alleviating aluminum(Al)toxicity in rice.【Method】The rice cultivar kasalath was selected as test material,and treated with 0μmol/L and 30μmol/L AlCl_(3),0.1mmol/L and 0.5 mmol/L CaCl_(2),0.2μmol/L NaHS and 100μmol/L H_(2)S scavenger hypotaurine(HP).The seeds were incubated at 30℃in the dark for 24 h,then the root elongation,total Al content,Al content in cell sap,Al content in apoplast,Al content in cell wall,pectin content,pectin methylesterase activity and the relative expression of OsSTAR2,Os NRAT1 and Os FRDL4 were measured to explore the molecular and physiological mechanism of the interaction between Ca^(2+)and H_(2)S alleviating Al toxicity in rice.【Result】Under Al stress,compared with 0.1 mmol/L CaCl_(2)treatment,0.5 mmol/L CaCl_(2)treatment significantly increased root elongation,H_(2)S content,total calcium(Ca)content and cytoplasm Ca content,and significantly decreased total Al content in root,cell sap Al content,apoplastic Al content and cell wall Al content in rice.The pretreatment of NaHS significantly increased the elongation of rice roots at the two calcium concentrations,accompanied with the significant increase of root tip Al content,root total Al content,cell sap Al content,apoplastic Al content and cell wall Al content.The relative expression levels of OsSTAR2 and Os FRDL4 were significantly increased and the relative expression of Os NRAT1 was significantly decreased at the two Ca^(2+)concentrations after pretreated with NaHS.However,the application of HP produced an opposite result.【Conclusion】Under Al stress,Ca^(2+)reduced the accumulation of Al in rice root cell wall and the absorption of Al by rice roots through increasing the production of H_(2)S in rice root,and finally alleviated the inhibition of Al on rice root elongation.

关 键 词：水稻 铝胁迫 钙离子 硫化氢 

分 类 号：S511[农业科学—作物学]