黄芪多糖对VEGF-C表达的抑制与结直肠癌细胞内淋巴管生成的相关性研究  

Correlation Study on the Inhibition of VEGF-C Expression by Astragalus Polysaccharides and Lymphangiogenesis in Colorectal Cancer Cells

作　　者：王奇钰 吴晗 唐立垚 王雅楠 高峰 WANG Qiyu;WU Han;TANG Liyao;WANG Ya'nan;GAO Feng(Hospital 940 of PLA Joint Logistics Support Force,Lanzhou 730050,China;Gansu Provincial Key Lab for Stem Cells and Gene Drugs,Lanzhou 730050,China;Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]解放军联勤保障部队第940医院,甘肃兰州730050 [2]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050 [3]甘肃中医药大学,甘肃兰州730000

出　　处：《西部中医药》2023年第3期47-51,共5页Western Journal of Traditional Chinese Medicine

基　　金：军后综〔2016〕号面上项目(16BJZ48)。

摘　　要：目的:了解黄芪多糖(astragalus polysaccharides,APS)作用后的人结直肠癌(colorectal cancer,CRC)细胞中血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)的表达,探讨其对CRC淋巴结转移的影响及可能机制。方法:对不同浓度APS处理后的LoVo细胞进行划痕实验,并应用Western印迹法和RT-PCR法分析VEGF-C/LYVE-1的表达。结果:与对照组相比,APS作用后的LoVo细胞中VEGF-C和LYVE-1的蛋白表达降低,且随浓度梯度降低(P<0.05)。与对照组相比,APS作用后的LoVo细胞中VEGF-C和LYVE-1基因的相对荧光表达量随浓度梯度降低,差异均有统计学意义(P<0.05)。结论:APS可能参与肿瘤淋巴管的生成,在CRC淋巴转移中发挥重要作用,且APS可能是一种潜在的肿瘤淋巴管生成的抗淋巴管生成剂,可用于预防和治疗CRC淋巴转移。Objective:To survey the expressions of VEGF-C in human colorectal cancer cells after the actions of APS,and explore its effects on lymph node metastasis in colorectal cancer and the possible mechanism.Methods:Scratch experiment was performed on LoVo cells treated with different concentrations of APS,Western blot and RT-PCR method were applied to analyze the expressions of VEGF-C/LYVE-1.Results:Compared with the control group,the expressions of VEGF-C and LYVE-1 protein reduced in LoVo cells after the actions of APS,and the protein relative expressions lowered when APS concentration reduced(P<0.05).Compared with the control group,the relative fluorescence expressions of VEGF-C and LYVE-1 genes in LoVo cells lowered after the actions of APS with the concentration gradient,and the difference had statistical meaning(P<0.05).Conclusion:APS might be involved in tumor lymphangiogenesis and play an important role in lymph node metastasis,and APS might be a potential anti-lymphangiogenic agent of tumor lymphangiogenesis,which could be used to prevent and treat lymphatic metastasis of colorectal cancer.

关 键 词：结直肠癌 淋巴结转移 VEGF-C/LYVE-1 黄芪多糖 

分 类 号：R273[医药卫生—中西医结合]