肠绞痛患儿血清sIL-2R、IFN-β、MIP-1β水平分析及与肠道菌群丰度的相关性  

Analysis of serum SIL-2R,IFN-β,MIP-1βlevels in children with colic and their correlation with intestinal microflora abundance

作　　者：高俊芳 Gao Junfang(Department of Gastroenterology,children's hospital affiliated to Zhengzhou University,Henan children's hospital,Zhengzhou children's hospital,Zhengzhou,Henan,450000,China)

机构地区：[1]郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院消化内科,河南郑州450000

出　　处：《齐齐哈尔医学院学报》2023年第2期106-111,共6页Journal of Qiqihar Medical University

基　　金：河南省医学科技攻关计划(LHGJ20190945)。

摘　　要：目的探讨肠绞痛患儿血清可溶性白细胞介素2受体(sIL-2R)、干扰素-β(IFN-β)、巨噬细胞炎性蛋白1β(MIP-1β)水平及与肠道菌群丰度的相关性。方法选择2020年1月—2022年1月本院收治的肠绞痛患儿120例作为观察组;另选择同期健康婴儿100名作为对照组。比较两组肠道菌群丰度、代谢指标、血清sIL-2R、IFN-β、MIP-1β水平差异。结果观察组双歧杆菌、韦荣球菌、萨特菌属、链球菌属和乳酸菌属丰度分别为0.79(0.68,0.92)、2.30(1.70,2.98)、0.77(0.62,0.90)、24.49(23.10,29.30)和11.10(9.30,12.80),明显高于对照组(P<0.05),而埃格特菌属、肠球菌属丰度分别为0.08(0.06,0.12)和5.10(4.30,6.70),明显低于对照组(P<0.05)。观察组乙酸为(3.19±0.92)mmol/L,明显低于对照组(P<0.05),丙酸、丁酸、异丁酸、戊酸、异戊酸分别为(1.20±0.21)mmol/L、(1.16±0.34)mmol/L、(1.23±0.19)mmol/L、(1.11±0.24)mmol/L和(1.09±0.21)mmol/L,明显高于对照(P<0.05)。观察组血清sIL-2R和MIP-1分别为(44.40±12.43)pg/ml和(110.40±31.12)pg/ml,明显高于对照组(P<0.05),而IFN-β为(33.10±11.43)pg/ml,明显低于对照组(P<0.05)。血清sIL-2R、MIP-1β与双歧杆菌属丰度呈正相关(P<0.05),而与肠球菌属丰度呈负相关(P<0.05)。血清IFN-β与双歧杆菌属丰度呈负相关(P<0.05),而与肠球菌属丰度呈正相关(P<0.05)。观察组治疗后双歧杆菌、韦荣球菌、萨特菌属、链球菌属、乳酸菌属丰度、丙酸、丁酸、异丁酸、戊酸、异戊酸、sIL-2R和MIP-1β明显低于治疗前(P<0.05),而埃格特菌属、肠球菌属丰度、乙酸、IFN-β明显高于治疗前(P<0.05)。结论肠绞痛患儿血清sIL-2R和MIP-1β水平升高,IFN-β水平降低,三者水平与双歧杆菌属、肠球菌属丰度呈相关性,值得进一步研究。Objective To investigate the levels of serum soluble interleukin-2 receptor(sIL-2R),interferon-β(IFN-β),macrophage inflammatory protein 1β(MIP-1β)and their correlation with intestinalmicroflora abundance in children with colic.Methods 120 children with colic those were treated in our hospitalfrom January 2020 to January 2022 were selected as the observation group,and 100 healthy infants were selectedas the control group,the intestinal flora abundance,metabolic indicators,serum sIL-2R,IFN-β,MIP-1βlevels were compared between the two groups.Results In the observation group,the abundance ofbifidobacteria,Veillonella,Sutterella,Streptococcus and Lactobacillus were 0.79(0.68,0.92),2.30(1.70,2.98),0.77(0.62,0.90),24.49(23.10,29.30)and 11.10(9.30,12.80),respectively,which weresignificantly higher than those of the control group(P<0.05),while the abundance of Egertella and Enterococcuswere 0.08(0.06,0.12)and 5.10(4.30,6.70),respectively,which were significantly lower than those of thecontrol group(P<0.05).The concentration of acetic acid in the observation group was(3.19±0.92)mmol/L,which was significantly lower than that in the control group(P<0.05).The concentrations of propionic acid,butyric acid,isobutyric acid,valeric acid and isovaleric acid were(1.20±0.21)mmol/L,(1.16±0.34)mmol/L,(1.23±0.19)mmol/L,(1.11±0.24)mmol/L and(1.09±0.21)mmol/L,respectively,which weresignificantly higher than those of control group(P<0.05).Serum sIL-2R and MIP-1 in the observation groupwere(44.40±12.43)pg/mL and(110.40±31.12)pg/ml,respectively,which were significantly higher thanthose in the control group(P<0.05);while IFN-was(33.10±11.43)pg/ml,which was significantly lower thanthat in the control group(P<0.05).Serum sIL-2R and MIP-1 were positively correlated with the abundance ofBifidobacterium(P<0.05),however,negatively correlated with the abundance of Enterococcus(P<0.05).Serum IFN-was negatively correlated with the abundance of Bifidobacterium(P<0.05),but positively correlatedwith the abundance of Enterococcus(P<0.05).I

关 键 词：肠绞痛 可溶性白细胞介素2受体 干扰素-Β 巨噬细胞炎性蛋白1β 肠道菌群丰度 

分 类 号：R725.7[医药卫生—儿科]