纳米薄膜基底对表面增强拉曼光谱检测环丙沙星的影响  

Effect of Nano-Film Substrates on Ciprofloxacin Detection by Surface-Enhanced Raman Spectroscopy

作　　者：郭义乾 王红艳 秦苗 曾一达 夏丽 胡扬坡 Guo Yiqian;Wang Hongyan;Qin Miao;Zeng Yida;Xia Li;Hu Yangpo(School of Aeronautical Manufacturing Engineering,Nanchang Hangkong University,Nanchang 330063,Jiangxi,China;School of Chemistry&Chemical Engineering,Suzhou University,Suzhou 234099,Anhui,China;Jiangxi Hongdu Aviation Industry Co.,Ltd.,Nanchang 330024,Jiangxi,China)

机构地区：[1]南昌航空大学航空制造工程学院,江西南昌330063 [2]宿州学院化学化工学院,安徽宿州234099 [3]江西洪都航空工业集团有限责任公司,江西南昌330024

出　　处：《激光与光电子学进展》2022年第23期207-216,共10页Laser & Optoelectronics Progress

基　　金：安徽省高校自然科学研究重点项目(KJ2020A0730,KJ2019A0669);国家自然科学基金(52105451);江西省重点研发计划(20202BBEL53029);轻合金加工科学与技术国防重点学科实验室开放课题研究基金(EG202003411)。

摘　　要：基于金纳米颗粒薄膜基底和金纳米棒薄膜基底,使用表面增强拉曼光谱(SERS)技术对环丙沙星(CIP)的含量进行了分析检测,为食品中CIP残留检测提供了新方法。通过使用柠檬酸钠还原氯金酸制备金纳米颗粒胶体,以及通过晶种生长法制备金纳米棒胶体,以应用于SERS增强基底。通过不同激发光波长对CIP进行SERS检测,确定了最佳激光波长为780 nm。使用校正集CIP标准溶液,建立CIP浓度-SERS信号强度的工作曲线,使用检验集样本观察工作曲线的预测能力。结果表明:使用金纳米颗粒基底进行CIP的SERS检测,回收率在97.1%~105.0%;使用金纳米棒基底进行SERS检测,回收率在96.3%~121.8%。因此,SERS在检测CIP抗生素领域具有高灵敏度、快速检测等优势。Based on gold nanoparticle and gold nanorod film substrates,surface-enhanced Raman spectroscopy(SERS)technology is used to detect the content of ciprofloxacin(CIP),providing a new mode of detecting ciprofloxacin residues in food.The gold nanoparticle colloid is prepared by using sodium citrate to reduce chloroauric acid,whereas the gold nanorod colloid is prepared by the seed growth method,and then applied to the SERS reinforced substrate.SERS detection of CIP is conducted using different excitation light wavelengths,and the optimal laser wavelength is determined to be 780 nm.The calibration-set CIP standard solution is used to establish a working curve of CIP concentration-SERS signal intensity,and the validation-set standard samples are used to observe the predictive ability.The results show that the recovery rates of SERS detection for CIP using gold nanoparticle and gold nanorod substrates are approximately 97.1%-105.0%and 96.3%-121.8%,respectively.This confirm that SERS has advantages of high sensitivity and rapid detection potential in the field of detecting CIP antibiotics.

关 键 词：医用光学与生物技术 表面增强拉曼光谱 环丙沙星 金纳米颗粒 金纳米棒 

分 类 号：O657.37[理学—分析化学]