miR-760靶向调节黑色素瘤抗原家族D1抑制胃癌细胞增殖、迁移及侵袭的研究  

Study on miR-760 targeting regulating melanoma antigen family D1 to inhibit the proliferation,migration and invasion of gastric cancer cells

作　　者：牛正宣[1] 周丽[2] 周大新[1] NIU Zheng-xuan;ZHOU Li;ZHOU Da-xin(Department of General Surgery,Huaibei People's Hospital,Huaibei 235100,Anhui Province,China;Department of Oncology,Chaohu Hospital of Anhui Medical University,Hefei 235000,Anhui Province,China)

机构地区：[1]淮北市人民医院普外科,安徽淮北235100 [2]安徽医科大学附属巢湖医院肿瘤内科,安徽合肥235000

出　　处：《中国临床药理学杂志》2023年第4期493-497,共5页The Chinese Journal of Clinical Pharmacology

基　　金：淮北市科技计划基金资助项目(rj201820)。

摘　　要：目的 探讨microRNA-760(miR-760)靶向负调节黑色素瘤抗原家族D1(NRAGE)表达对胃癌细胞增殖、迁移及侵袭的作用。方法 将胃癌NCI-N87细胞分为空白组、miR-760 NC组、miR-760 mimics组、miR-760 mimics+NRAGE NC组和miR-760 mimics+NRAGE OE组。空白组用RPMI 1640培养基培养;miR-760 NC组、miR-760 mimics组分别转染miR-760 NC、miR-760 mimics;miR-760 mimics+NRAGE NC组同时转染miR-760 mimics和空载pcDNA3.1质粒;miR-760 mimics+NRAGE OE组同时转染miR-760 mimics和NRAGE过表达pcDNA3.1质粒。用蛋白质印迹法检测NRAGE的表达水平,用Transwell实验法检测NCI-N87细胞的迁移、侵袭能力。结果 空白组、miR-760 NC组和miR-760 mimics组的NRAGE蛋白相对表达水平分别为0.95±0.06,0.93±0.09和0.27±0.04;迁移细胞数分别为(268.65±14.08),(261.43±13.25)和(163.25±11.37)个;侵袭细胞数量分别为(183.58±10.65),(176.29±9.93)和(95.73±8.29)个;miR-760 mimics组的上述指标均较空白组、miR-760 NC组显著降低(均P<0.05)。NRAGE OE可显著减弱miR-760 mimics对上述指标的影响(均P<0.05)。结论 miR-760可能通过靶向下调NRAGE表达,抑制胃癌NCI-N87细胞的增殖、迁移及侵袭。Objective To investigate the effect of microRNA-760(miR-760) targeting and negatively regulating the expression of melanoma antigen family D1(NRAGE) on the proliferation,migration and invasion of gastric cancer cells.Methods The gastric cancer NCI-N87 cells were divided into blank group,miR-760 NC group,miR-760 mimics group,miR-760 mimics +NRAGE NC group and miR-760 mimics+NRAGE OE group.The blank group was cultured with RPMI 1640 medium.miR-760 NC group and miR-760 mimics group were transfected with miR-760 NC and miR-760 mimics,respectively.miR-760 mimics+NRAGE NC group was transfected with both miR-760 mimics and empty pcDNA3.1 plasmid.760 mimics+NRAGE OE group was transfected with both miR-760 mimics and NRAGE overexpression pcDNA3.1 plasmid.Western blot were used to detect the expression of NRAGE.Transwell test was used to detect the migration and invasion ability of NCI-N87 cells.Results The relative expression levels of NRAGE protein in the blank,miR-760 NC and miR-760 mimics groups were 0.95 ± 0.06,0.93 ± 0.09 and 0.27 ± 0.04;the number of migrating cells was 268.65 ± 14.08,261.43 ± 13.25 and163.25 ± 11.37;the number of invasive cells was 183.58 ± 10.65,176.29 ± 9.93 and 95.73 ± 8.29,respectively.The above indicators in the miR-760 mimics group were all significantly lower than those in blank group and miR-760 NC group( all P < 0.05).NRAGE OE significantly attenuated the effect of miR-760 mimics on the above indexes( all P < 0.05).Conclusion miR-760 can inhibit the proliferation,migration and invasion of gastric cancer NCI-N87 cells by targeting down-regulation of NRAGE.

关 键 词：胃癌 microRNA-760 黑色素瘤抗原家族D1 增殖 迁移 侵袭 

分 类 号：R97[医药卫生—药品]