苏木及其活性成分对耐碳青霉烯类鲍曼不动杆菌的体外抑制作用研究  

Inhibitory effect of hematoxylon and its active components on carbapenem-resistant Acinetobacter baumannii in vitro

作　　者：徐令清[1] 杜良琴 袁润奇 晏嘉 陈旋 简永欢 周飘雁 温伟洪[1] 李林海 XU Lingqing;DU Liangqin;YUAN Runqi;YAN Jia;CHEN Xuan;JIAN Yonghuan;ZHOU Piaoyan;WEN Weihong;LI Linhai(Department of Laboratory,the Sixth Affiliated Hospital of Guangzhou Medical University/Qingyuan People’s Hospital,Qingyuan,Guangdong 511518,China)

机构地区：[1]广州医科大学附属第六医院/清远市人民医院检验科,广东清远511518

出　　处：《重庆医学》2023年第5期662-666,671,共6页Chongqing medicine

基　　金：广东省中医药局项目(20201407);广东省科技创新战略专项资金(DZXQY002);广东省医学科学技术研究基金(A2021490);广东省清远市科技计划项目(200808114560452);广东省清远市人民医院医学科研基金支持项目(20190209);广州医科大学第六临床学院大学生创新项目(2021ALY06)。

摘　　要：目的探讨苏木及其活性成分对耐碳青霉烯类鲍曼不动杆菌(CRABA)的杀菌作用及巴西苏木素与美罗培南联合抑菌作用。方法收集2020年该院检验科微生物室分离的10株CRABA,采用微量肉汤稀释法测定苏木萃取液和巴西苏木素的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),采用高效液相色谱技术测定苏木萃取液的主要抑菌组分,采用绘制生长曲线方法观察巴西苏木素对CRABA生长的抑制作用,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳测定巴西苏木素对CRABA分泌蛋白的影响,棋盘法观察巴西苏木素与美罗培南联合应用的抑菌效果。结果苏木萃取液对10株CRABA的MIC、MBC均为15.63 mg/mL,巴西苏木素的MIC、MBC均为0.5000 mg/mL;高效液相色谱法和MIC测定得到巴西苏木素为苏木萃取液中主要抑菌组分。巴西苏木素与美罗培南联用时,巴西苏木素的MIC值由0.5000 mg/mL降至0.1250 mg/mL,美罗培南的MIC值由0.128 mg/mL降至0.064 mg/mL,FIC指数为0.75。结论苏木中有效抑菌组分为巴西苏木素,其机制可能是通过影响细菌蛋白的分泌发挥杀菌作用。Objective To explore the bactericidal effect of hematoxylon and its active components on carbapenem-resistant Acinetobacter baumannii(CRABA)and the combined antibacterial effect of Brazilian hematoxylin and meropenem.Methods A total of 10 CRABA strains were collected.Minimal inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of hematoxylin extract and Brazilian hematoxylin were determined by the micro broth dilution method.High-performance liquid chromatography(HPLC)was used to determine the main antibacterial components of hematoxylin extract.Growth curve was used to observe the inhibitory effect of Brazilian hematoxylin on the growth of CRABA.Sodium dodecyl sulfonate-polyacrylamide gel electrophoresis(SDS-PAGE)was used to determine the effect of Brazilian hematoxylin on the secreted protein of CRABA.The checkerboard method was used to observe the antibacterial effect of the combined application of Brazilian hematoxylin and meropenem.Results The MIC value and MBC value of hematoxylin extract to 10 CRABA strains were 15.63 mg/mL;the MIC value and MBC value of Brazilian hematoxylin to 10 CRABA strains were 0.5000 mg/mL.Brazilian hematoxylin is obtained as the main antibacterial component in hematoxylin extract by high performance liquid chromatography.When Brazilian hematoxylin was combined with meropenem,the MIC value of Brazilian hematoxylin decreased from 0.5000 mg/mL to 0.1250 mg/mL,and the MIC value of meropenem decreased from 0.128 mg/mL to 0.064 mg/mL,and the FIC index was 0.75.Conclusion The effective antibacterial component in hematoxylon is Brazilian hematoxylin,which may exert a bactericidal effect by affecting the secretion of bacterial proteins.

关 键 词：苏木 巴西苏木素 原苏木素B 耐碳青霉烯类鲍曼不动杆菌 美罗培南 

分 类 号：R378.2[医药卫生—病原生物学]