白英总皂苷的抗炎作用及机制研究  

The effect and mechanism of total saponins from Solanum lyratum Thunb in anti-inflammatory

作　　者：马金理 廖诗语 李宛泽 杨文博 金丹丹 费瑞 杜建时[1,2] MA Jin-li;LIAO Shi-yu;LI Wan-ze;YANG Wen-bo;JIN Dan-dan;FEI Rui;DU Jian-shi(China-Japan Union Hospital of Jilin University,Changchun 130033,China;Key Laboratory of Lymphatic Surgery Jilin Province,Changchun 130031,China;School of Basic Medical Science,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林省淋巴外科重点实验室,吉林长春130031 [3]吉林大学基础医学院,吉林长春130021

出　　处：《东北师大学报（自然科学版）》2023年第1期117-122,共6页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20180101112JC).

摘　　要：利用脂多糖刺激RAW 264.7细胞(小鼠单核巨噬细胞系)产生的炎性反应,检测了白英总皂苷对RAW264.7细胞的TNF-α、MD2和TLR4蛋白表达的影响,探讨了白英总皂苷在炎性反应中的作用及分子机制.结果表明:白英总皂苷对RAW264.7细胞无毒性作用,但对TNF-α蛋白表达有抑制作用,且这一过程可通过抑制MD2和TLR4蛋白的表达实现.In order to further explore the role and molecular mechanism of total saponins in inflammatory response,the effects of total saponins on the expression of TNF-α,MD2 and TLR4 proteins were detected on the basis of the inflammatory response of RAW264.7 cells stimulated by Lipopolysaccharide(LPS).The results showed that the total saponins had no toxic effect on RAW264.7 cells,but had inhibitory effect on the expression of TNF-α,which might be realized by inhibiting the expression of MD2 and TLR4 proteins.The results of this study provide the experimental basis for the further development of the anti-inflammatory candidate drugs of the total saponins.

关 键 词：白英总皂苷 炎症 RAW264.7细胞 MD2 TLR4 

分 类 号：R966[医药卫生—药理学]