不同类型冬水田土壤中厌氧甲烷氧化古菌的垂直分布  

Vertical Distribution of Anaerobic Methane-oxidizing Archaea in Different Types of Flooded Paddy Field

作　　者：余子洁 申思 郭大陆 李致同 罗红燕[1] YU Zijie;SHEN Si;GUO Dalu;LI Zhitong;LUO Hongyan(College of Resources and Environment,Southwest University,Chongqing 400715,China;Southwest University Science Park,Chongqing 400700,China)

机构地区：[1]西南大学资源环境学院,重庆400715 [2]重庆市北碚国家大学科技园,重庆400700

出　　处：《江西农业大学学报》2023年第1期220-230,共11页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(41301315)。

摘　　要：【目的】厌氧甲烷氧化古菌是厌氧条件下减少甲烷排放的关键微生物,在全球碳素循环中发挥着重要作用,但其在不同类型冬水田土壤中的垂直分布规律尚不清楚。【方法】利用qPCR和16S rRNA测序技术,选择厌氧甲烷氧化古菌的特异性引物DP397F(q)/DP569R(q)和DP142R/DP779R,探究潴育型和潜育型冬水田的耕作层、犁底层和潴育层/潜育层中厌氧甲烷氧化古菌的丰度和生态位分布。【结果】两种类型水稻土中存在大量厌氧甲烷氧化古菌,且古菌丰度值在4.82×106~9.30×107copies/g干土,菌群丰度及其多样性水平均呈现出潴育层/潜育层>犁底层>耕作层。相关性分析表明,两种类型冬水田厌氧甲烷氧化古菌丰度均与pH值呈显著正相关(P<0.05),与SOM、WC和NH4+-N呈显著负相关(P<0.05);厌氧甲烷氧化古菌氧化速率均与SOM、NH4+-N呈显著负相关(P<0.05)。【结论】潴育型冬水田厌氧甲烷氧化古菌多样性高于潜育型冬水田厌氧甲烷氧化古菌多样性,但厌氧甲烷氧化古菌丰度值则相反。两个采样点厌氧甲烷氧化古菌群落结构多样性和丰度值均呈现出随土层深度增加而增加。厌氧甲烷氧化反应在全球甲烷调控和减少海洋和淡水生态系统甲烷释放方面发挥着重要作用,对全球碳素循环具有重要意义。[Objective]Anaerobic methane-oxidizing archaea(ANME)are the key microorganisms to reduce methane emissions under anaerobic conditions and play an important role in the global carbon cycle.However,the vertical distribution of anaerobic methane-oxidizing archaea in different types of flooded paddy fields is unclear.[Method]Using qPCR and 16S rRNA sequencing technology,the specific primers DP397F(q)/DP569R(q)and DP142R/DP779R of ANME were selected to explore the abundance and niche distribution of ANME in the arable layer,plow bottom layer and waterloggogenic/gley layer of flooded paddy field.[Result]There were a large number of ANME in the two types of flooded paddy filed,and the abundance of archaea was between 4.82×10~6-9.30×10~7 copies/g dry soil.The abundance and diversity of archaea showed the trend of waterloggogenic/gley>plow bottom layer>arable layer.The abundance of ANME in the two types of flooded paddy field were in a significantly positive correlation with pH(P<0.05),and in a significantly negative correlation with SOM,WC and NH4+-N(P<0.05).The oxidation rates of ANME were significantly negative correlation with SOM and NH4+-N(P<0.05).[Conclusion]Diversity of ANME in waterloggogenic paddy fields was higher than that in gley paddy fields,but the abundance was opposite.The diversity and abundance of anaerobic methane oxidizing archaea community structure increased with the increase of soil depth.Anaerobic methane oxidation plays a vital role in global methane regulation and reduction of methane release from marine and freshwater ecosystems,and is of great significance to the global carbon cycle.

关 键 词：厌氧甲烷氧化古菌 潴育型 潜育型 冬水田 

分 类 号：S182[农业科学—农业基础科学]