原发性高血压合并乙型肝炎病毒感染患者Rho/ROCK通路研究  

Rho/ROCK Pathway in Patients with Essential Hypertension Complicated with Hepatitis B Virus Infection

作　　者：杨柳[1] 曾繁颖 谭昀 黄颖梅[1] 欧阳长生[1] 胡林[1] 王洪[1] Yang Liu;Zeng Fan-ying;Tan Yun;Huang Ying-mei;Ouyang Chang-sheng;Hu Lin;Wang Hong(The First Affiliated Hospital of Nanchang Medical College,Jiangxi Provincial People's Hospital,Nanchang 330006,Jiangxi Province,China;Lianhua County People's Hospital,Pingxiang 337000,Jiangxi Province,China;Jinxian County People's Hospital,Nanchang 330000,Jiangxi Province,China)

机构地区：[1]南昌医学院第一附属医院,江西省人民医院,江西南昌330006 [2]江西省萍乡市莲花县人民医院2,江西萍乡337000 [3]江西省南昌市进贤县人民医院3,江西南昌330000

出　　处：《中外医药研究》2022年第14期6-8,共3页JOURNAL OF CHINESE AND FOREIGN MEDICINE AND PHARMACY RESEARCH

基　　金：江西省卫生健康委科技计划项目(编号:20203009);南昌医学院第一附属医院江西省人民医院博士启动(编号:19-236号)。

摘　　要：目的:探讨原发性高血压患者中乙型肝炎感染患者比例及血管内皮生长因子(VEGF)通过ROCK信号通路升高血压的效应。方法:选取2020年1月-2022年1月南昌医学院第一附属医院收治的原发性高血压患者和同期健康人群各200例为观察对象。检测HBsAg、抗HBc、抗HBs、抗HCV,测定乙型肝炎患者乙肝病毒脱氧核糖核酸(HBV-DNA)和外周血VEGF水平。建立人脐静脉内皮细胞(HUVEC)高血压损伤模型,将细胞分为对照组、AngⅡ模型空白组、AngⅡ+VEGF组。结果:高血压组患乙型肝炎比例高于非高血压组,差异有统计学意义(P<0.05);8例乙型肝炎患者的HBV-DNA拷贝数与VEGF水平呈正相关(r=0.949);在AngⅡ+VEGF组中,ROCK1、ROCK2表达水平明显上调。结论:乙型肝炎病毒感染在高血压患者中比例较高,VEGF水平随乙型肝炎患者体内HBV-DNA的拷贝数增高而增高。Objective:To investigate the proportion of patients with hepatitis B infection and the effect of vascular endothelial growth factor(VEGF)on blood pressure elevation through ROCK signaling pathway in patients with essential hypertension.Methods:A total of two hundred patients with essential hypertension admitted to the First Affiliated Hospital of Nanchang Medical College from January 2020 to January 2022 and two hundred healthy people in the same period were selected as the observation objects.HBsAg,anti-HBc,anti-HBs and anti-HCV were detected.The levels of hepatitis B virus deoxyribonucleic acid(HBVDNA)and peripheral blood VEGF in patients with hepatitis B were determined.Human umbilical vein endothelial cell(HUVEC)model of hypertension was established.The cells were divided into control group,AngⅡmodel blank group and AngⅡ+VEGF group.Results:The proportion of hepatitis B in hypertensive group was higher than that in non-hypertensive group,and the difference was statistically significant(P<0.05).HBV-DNA copy number was positively correlated with VEGF level in 8 patients with hepatitis B(r=0.949).The expression levels of ROCK1 and ROCK2 were significantly up-regulated in AngⅡ+VEGF group.Conclusion:The proportion of hepatitis B virus infection was higher in hypertensive patients,and the level of VEGF increased with the increase of HBV-DNA copy number in hepatitis B patients.

关 键 词：乙型肝炎 原发性高血压 人脐静脉内皮细胞 Rho/ROCK通路 

分 类 号：R51[医药卫生—内科学]