妊娠期糖尿病与高脂血症孕妇肠道菌群构成特征比较  

Composition and differences between gestational diabetes mellitus and hyperlipidemia during pregnancy

作　　者：邱鸿晖 张东耀 苏尧 陈莉[1] 曹艳楠 徐先明[1] QIU Hong-hui;ZHANG Dong-yao;SU Yao;CHEN Li;CAO Yan-nan;XU Xian-ming(Department of Obstetrics and Gynecology,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201620,China)

机构地区：[1]上海交通大学医学院附属第一人民医院妇产科,上海201620

出　　处：《川北医学院学报》2023年第2期203-207,共5页Journal of North Sichuan Medical College

基　　金：上海申康医院发展中心促进市级医院临床技能与临床创新能力三年行动计划重大临床研究项目(SHDC2020CR^(2)060B)。

摘　　要：目的:探讨妊娠期糖尿病与妊娠合并高脂血症的肠道菌群构成特点,寻找两种妊娠代谢性疾病的肠道菌群相关的潜在致病机制。方法:收集妊娠期糖尿病孕妇24例、妊娠合并高脂血症孕妇23例、健康产检孕妇17名,采集粪便并借助16S rDNA扩增子测序技术进行测序,借助QIIME及R语言软件平台,分析三类样本中肠道的菌群在Alpha多样性、Beta多样性、LEfSe的差异性方面的生物学信息。结果:妊娠期糖尿病组Alpha多样性较妊娠合并高脂血症组偏低(Shannon的指数值为3.78±0.73、4.38±0.75,t=3.001,P=0.005;Chao1指数为815.38±376.43,1107.56±489.52,t=2.676,P=0.008);妊娠期糖尿病组的Alpha多样性低于对照组(P<0.05),高脂血症组与对照组的Alpha多样性比较,差异无统计学意义(P>0.05)。妊娠期糖尿病组与高脂血症组的Beta多样性比较,差异有统计学意义(R^(2)=0.03,P=0.001),妊娠期糖尿病组与对照组的Beta多样性比较,差异有统计学意义(R^(2)=0.04,P=0.034),高脂血症组与对照组相比Beta多样性比较,差异有统计学意义(R^(2)=0.04,P=0.018)。妊娠期糖尿病组、高脂血症组在属水平的肠道菌群差异:Firmicutes、Bacteroidetes、Proteobacteria是相对丰度最高的三大菌群,两组间的相对丰度比较,差异无统计学意义(P>0.05);在属水平,Blautia、Dorea、Akkermansia、Bifidobacterium的相对丰度比较,差异无统计学意义(P>0.05)。结论:与妊娠期糖尿病组相比,高脂血症组与正常对照组的菌群组成和构成更相似,而妊娠期糖尿病患者与妊娠中期高脂血症患者在菌属构成和相对丰度有关键菌群的重合,预示着二者可能存在共同的菌群代谢机制,参与介导糖代谢、脂代谢及胰岛素抵抗。Objective:To investigate the characteristics of gut microbiome composition between GDM and gestational combined hyperlipidemia, and to search for potential pathogenic mechanisms related to gut microbiota in two metabolic diseases of pregnancy.Methods:Based on 16SrDNA amplicon sequencing technology, 24 pregnant women with GDM,23 pregnant women with combined hyperlipidemia of pregnancy and 17 healthy pregnant women were subjected to stool collection and sequencing, and the QIIME software platform was used to perform Alpha diversity, Beta diversity and LEfSe differences of gutmicrobiota in the three groups of samples.Results:Alpha diversity of the gestational diabetes group was lower than that of the gestational combined hyperlipidemia group(Shannon index was 3.78±0.73,4.38±0.75,t=3.001,P=0.005,Chao1 index was 815.38±376.43,1107.56±489.52,t=2.676,P=0.008),Alpha diversity of the GDM was lower than that of the control group, and there was no significant difference between the hyperlipidemia group and the control group.Beta diversity was statistically different between GDM group and hyperlipidemia group(R^(2) =0.03,P=0.001),Beta diversity was statistically different between GDM group and control group(R^(2)=0.04,P=0/034),Beta diversity was statistically different between hyperlipidemia group compared with control group(R^(2) =0.04,p=0.018).At the genus level, Firmicutes, Bacteroidetes, and Proteobacteria were the three groups with the highest relative abundance both in the GDM and hyperlipidemia groups, and the relative abundance was not statistically different between the two groups(P>0.05),at the genus level, Blautia, Dorea, Akkermansia, and Bifidobacterium were not statistically different in relative abundance(P>0.05).Conclusion:The composition of the microbiota are more similar between the hyperlipidemic group and the normal control group compared to the GDM group, while there is an overlap in the genus composition and relative abundance of key microbiota between patients with GDM and those with hyperlipidemia

关 键 词：糖尿病 高脂血症 肠道菌群 妊娠 代谢综合征 

分 类 号：R714.2[医药卫生—妇产科学] R714.7[医药卫生—临床医学]