狗蚁草总生物碱对大鼠肝星状细胞胶原蛋白、细胞因子的影响  

Effects of total alkaloids from Alysicarpus vaginalis L.DC.on the collagen and cytokines of rat hepatic stellate cells

作　　者：唐云丽 郑作文 甘彩玉 梁冰洁 韦建华 张文涛 彭成[1] TANG Yun-li;ZHENG Zuo-wen;GAN Cai-yu;LIANG Bing-jie;WEI Jian-hua;ZHANG Wen-tao;PENG Cheng(State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,Chengdu University of Traditional Chinese Medicine,Chengdu611137,China;College of Pharmacy,Guangxi University of Chinese Medicine,Nanning530200,China)

机构地区：[1]成都中医药大学西南特色中药资源国家重点实验室,成都611137 [2]广西中医药大学药学院,南宁530200

出　　处：《湖北农业科学》2023年第1期134-138,共5页Hubei Agricultural Sciences

基　　金：广西科技基地和人才专项(桂科AD20238058);中药学学科建设(2017年中央财政支持地方高校专项)(05017105);广西高校科技创新能力提升工程专项“中药药理重点实验室”(J15185)。

摘　　要：以胶原蛋白、细胞因子为考察对象,探索狗蚁草(Alysicarpus vaginalis L.DC.)总生物碱(AVTA)的抗纤维化作用机制。采用MTT法检测AVTA抑制肝星状细胞增殖的无毒浓度;采用酶联免疫吸附法(ELISA)检测AVTA(10.00、7.00、4.90μg/mL)对T6细胞上清液COLⅠ(Ⅰ型胶原)、COLⅢ(Ⅲ型胶原)和PDGF(血小板衍生生长因子)、IGF-1(胰岛素样生长因子-1)、TNF-α(肿瘤坏死因子)及IL-6(白细胞介素-6)含量的影响。结果表明,AVTA抑制肝星状细胞增殖的无毒浓度在13.72μg/mL以下;与细胞对照组相比,10.00μg/mL的AVTA能显著抑制COLⅠ、COLⅢ、PDGF、TNF-α、IGF-1、IL-6的分泌(P<0.05或P<0.01)。The anti-fibrosis mechanism of total alkaloids of Alysicarpus vaginalis(AVTA)collagen and cytokines were investigated.MTT assay was used to detect the non-toxic concentration of AVTA inhibiting the proliferation of hepatic stellate cells.And the effect of AVTA(10.00,7.00,4.90μg/mL)on the content of typeⅠcollagen(COLⅠ),typeⅢcollagen(COLⅢ)and platelet-derived supernatant fluid growth factor(PDGF),tumor necrosis factor(TNF-α),insulin-like growth factor 1(IGF 1)and Interleukin 6(IL-6)were detected in T6 cells by ELISA.Results showed that the non-toxic concentration of AVTA inhibiting the proliferation of hepatic stellate cells was less than 13.72μg/mL;Compared with the cells in the control group,the total alkaloid 10.00μg/mL could inhibit the secretion of COLⅠ,COLⅢ,PDGF,TNF-α,IGF-1,IL-6(P<0.05 or P<0.01).

关 键 词：狗蚁草(Alysicarpus vaginalis L.DC.)总生物碱 HSC-T6 胶原蛋白 细胞因子 

分 类 号：R285.5[医药卫生—中药学]