基于转录组测序方斑东风螺LPS刺激的免疫机制研究  

Exploration Related to Immune Mechanism of Babylonia areolata in Response to LPS Challenged Based on Transcriptome Sequencing

作　　者：陈宗发 洪雨洁 赵明明 王菁 王忠良[1] CHEN Zong-fa;HONG Yu-jie;ZHAO Ming-ming(Fisheries College,Guangdong Ocean University,Zhanjiang,Guangdong 524088)

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《安徽农业科学》2023年第4期85-91,共7页Journal of Anhui Agricultural Sciences

基　　金：广东省省级科技计划项目(国际科技合作领域)(2019A050510044);2021年度广东省普通高校特色创新类项目(2021KTSCX044);广东省自然科学基金项目(2019A1515011875);广东海洋大学2019年“冲一流”省财政专项资金建设项目(校〔2019〕21号);国家级大学生创新创业训练计划项目(CXXL2020002);广东海洋大学“南海学者计划”(2017年度);广东海洋大学2021年度本科生创新团队项目(CXTD2021001)。

摘　　要：[目的]进一步挖掘方斑东风螺(Babylonia areolata)免疫系统中参与响应脂多糖刺激的重要功能基因与通路。[方法]采用Illumina HiSeqTM 2000高通量测序平台对不同处理条件的方斑东风螺进行测序分析。所得的原始数据经质控、组装后分别比对到KOG、Nr、Swiss-Prot和KEGG数据库进行功能注释,筛选差异表达基因并进行聚类分析。[结果]GO功能注释结果显示,多数unigenes被注释到代谢过程(metabolic process)、催化活性(catalytic activity)和细胞过程(cellular process)。KEGG注释结果显示,多数差异表达基因集中于代谢通路(Metabolic pathways)(28.3%)、次生代谢物的生物合成(Biosynthesis of secondary metabolites)(8.69%)、核糖体(Ribosome)(7.47%)和内吞作用(Endocytosis)(5.84%)。对比对照组,试验组中LPS刺激后4 h组具有9096个差异表达基因,8 h组具有10335个差异表达基因,而4 h组和8 h组间则存在8756个差异表达基因,通过KEGG数据库对DEGs进行注释和功能富集,关注到的显著通路包括NOD样受体信号通路、趋化因子信号通路等。[结论]暗示脂多糖调节方斑东风螺的抗病能力通过许多通路,是一个复杂的过程,可为进一步研究方斑东风螺的免疫响应机制研究提供理论数据。[Objective]To further explore the important functional genes and pathways involved in the immune mechanism stimulated by LPS.[Method]The transcriptomes of foot of Babylonia areolata were sequenced through high-throughput sequencing technology.After trimming and filtering,the original data were compared to KOG,Nr,Swiss-Prot and KEGG databases for functional annotation and differential gene cluster analysis.[Result]The results of GO functional annotation showed that most of the unigenes were annotated to metabolic process,catalytic activity and cellular process.KEGG pathway annotations showed that most differentially expressed genes were mainly concentrated in Metabolic pathway(28.3%),biosynthesis of secondary metabolites(8.69%),ribosome(7.47%)and endocytosis(5.84%).Compared with the control group,there were 9096 differentially expressed genes in the experimental group at 4 h after LPS stimulation,10335 differentially expressed genes at 8 h after LPS stimulation,and 8756 differentially expressed genes at 4 h and 8 h after LPS stimulation.DEGs was annotated and functionally enriched by KEGG database,including nod-like receptor signal pathway,chemokine signal pathway and so on.[Conclusion]The results of this study enrich the genetic resources of Babylonia areolata,and provide basic theoretical data for further study of the immune response mechanism of Babylonia areolata.

关 键 词：方斑东方螺 转录组 脂多糖 免疫通路 

分 类 号：S917.4[农业科学—水产科学]