两个马铃薯Y病毒不同株系分离物在三种寄主上的致病性分析  

Pathogenicity and virulence of two different PVY isolates associated with three different hosts

作　　者：刘晴 姜新 刘春菊[3] 郭永良[3] 闫志勇 耿超[1] 王杰[4] 徐蓬军[4] 李向东[1,2] 田延平 LIU Qing;JIANG Xin;LIU Chunju;GUO Yongliang;YAN Zhiyong;GENG Chao;WANG Jie;XU Pengjun;LI Xiangdong;TIAN Yanping(Department of Plant Pathology,College of Plant Protection,Shandong Agricultural University,Tai’an 271018,China;Shandong Provincial Key Laboratory of Agricultural Microbiology,Tai’an 271018,China;Shandong Weifang Tobacco Company,Weifang 261031,China;Tobacco Research Institute of CAAS,Qingdao 266101,China)

机构地区：[1]山东农业大学植物保护学院植物病理学系,泰安271018 [2]山东省农业微生物重点实验室,泰安271018 [3]山东潍坊烟草有限公司,潍坊261031 [4]中国农业科学院烟草研究所,青岛266101

出　　处：《植物病理学报》2023年第1期13-21,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31871933、31501612);山东省烟草公司项目(KN275);山东潍坊烟草有限公司(2021-47)。

摘　　要：马铃薯是重要的粮食和经济作物。马铃薯Y病毒(potato virus Y,PVY)是危害马铃薯安全生产的重要病毒。近年来,危害我国马铃薯的PVY株系组成发生了显著变化。PVY重组型株系尤其是PVY^(NTN-NWSYRI)和SYRII型成为优势株系,但与传统株系分离物相比,优势株系分离物在不同寄主上的侵染性及其致病力还不清楚。本研究分析了PVYN株系代表性分离物PVY^(N)605和PVY^(NTN-NWSYRI)型分离物GZ在本氏烟、普通烟和辣椒上的侵染性,比较了二者在本氏烟和普通烟上的致病力。结果表明,PVY^(N)605和PVY^(NTN-NWSYRI)-GZ分离物均能侵染本氏烟和普通烟,并在普通烟上引起叶脉坏死;PVY^(N)605不能系统侵染辣椒品种‘特大牛角王’,而PVY^(NTN-NWSYRI)-GZ可系统侵染辣椒品种特大牛角王。PVY^(NTN-NWSYRI)-GZ在本氏烟细胞间的移动速度明显慢于PVY^(N)605,但引起本氏烟矮化程度强于PVY^(N)605。截至接种后第11天,PVY^(NTN-NWSYRI)-GZ在系统叶片的积累水平明显低于PVY^(N)605。研究结果对了解PVY种群变化及制定针对性防控策略具有重要意义。Potato is one of the most important food and economic crops.Potato virus Y is an economically important virus affecting potato production.Recent studies have shown that the composition of PVY strain groups has changed.Recombinant PVY isolates including PVY^(NTN-NWSYRI)and PVY^(NTN-NWSYRI)I have become the predominant strains.However,their pathogenicity and virulence were unclear compared with previous PVYNstrain isolates.In this study,we compared the infectivity of isolates PVY^(N)605 and PVY^(NTN-NWSYRI)-GZ(originally isolated from tobacco plant)on Nicotiana benthamiana,N.tabacum and Capsicum annuum(Tedaniujiaowang)plants and their virulence on the plants of N.benthamiana and N.tabacum.Results showed that both isolates PVY^(N)605 and PVY^(NTN-NWSYRI)-GZ could infect N.benthamiana and N.tabacum systemically and induced vein necrosis in N.tabacum.Isolate PVY^(NTN-NWSYRI)-GZ,but not PVY^(N)605,could infect C.annuum(Tedaniujiao-wang)systemically.The cell-to-cell movement of PVY^(NTN-NWSYRI)-GZ is slower than that of PVY^(N)605.The reduction in the height of N.benthamiana plants caused by PVY^(NTN-NWSYRI)-GZ was more severe than that by PVY^(N)605.At 11 days post agroinfiltration,the accumulation level of PVY^(NTN-NWSYRI)-GZ in N.benthamiana plants was lower than that of PVY^(N)605.The results obtained in this study will help us understand the evolution of the PVY strains and design a new control strategy for PVY.

关 键 词：马铃薯Y病毒 致病性 致病力 株系组 重组 

分 类 号：S432.41[农业科学—植物病理学]