TeMV与PWV侵染对西番莲代谢生理及组织微结构的影响  

Effects of TeMV and PWV infection on metabolic physiology and tissue microstructure of passion fruit

作　　者：黄诚梅[1] 胡春锦[2] 史国英[2] 罗海斌[1] 曹辉庆[1] 吴兴剑 蒋胜理 叶丽萍 魏源文[1,3] HUANG Chengmei;HU Chunjin;SHI Guoying;LUO Haibin;CAO Huiqing;WU Xingjian;JIANG Shengli;YE Liping;WEI Yuanwen(Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China;Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Agro-products Quality Safety and Testing Technology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]广西农业科学院微生物研究所,南宁530007 [3]广西农业科学院农产品质量安全与检测技术研究所,南宁530007

出　　处：《植物病理学报》2023年第1期22-30,共9页Acta Phytopathologica Sinica

基　　金：广西农业科学院基本科研业务专项(2021YT118、桂农科2020YM103、2021JM99)。

摘　　要：以西番莲品种‘台农1号’为实验材料,通过电镜扫描技术比较观测TeMV和PWV侵染与健康西番莲的叶片、幼嫩果实外果皮组织形态结构,分析测定其叶片生理生化变化,研究西番莲病毒侵染对西番莲叶片和幼嫩果实外果皮组织结构和生理生化的影响。结果表明:TeMV与PWV侵染后,西番莲植株叶片的海绵组织细胞间隙缩小,发生成团堆积状排列,栅栏组织细胞萎缩,呈不规则长条形;幼嫩果实外表皮组织中石细胞层发生严重断裂,排列松散,海绵层细胞变得松散,且发生较严重的木质化现象;叶片与幼嫩果实外表皮表面纹理粗糙、褶皱,气孔保卫细胞萎缩,气孔周围组织皱缩、粗燥;感病果实外果皮表面上的腺毛凸起无序。病毒侵染后,感病植株第1~3叶的蔗糖含量较第4~6叶的高23.11%;健康叶片则相对平衡,降低了淀粉积累、PG酶、纤维素酶与PPO酶活性,提高了感病植株第1~3叶的α-淀粉酶、GS酶与POD酶活性。西番莲受TeMV与PWV侵染后,植株叶片第1~6叶营养物质分配不平衡,相关物质的合成分解与抗氧化防御酶系统受伤害,叶片与果实组织形态结构受到不同程度的损坏,植株正常吸收功能遭到破坏。Passion fruit variety ’Tainong 1’ was used as experimental material. The morphological structure of leaves and fruit exocarp of healthy and TeMV and PWV-infected plants was observed by scanning electron microscope. The physiological and biochemical changes of leaves were determined, and the effects of passion fruit virus infection on the morphological structure of leaves and young fruit exocarp, physiology and biochemistry were studied. The results showed that after TeMV and PWV infection, the spongy tissue cells of passion fruit leaves arranged in clusters with small spaces, and the palisade tissue cells atrophied in an irregular strip shape. The stone cell layer in the outer epidermis of young fruit was severely damaged and arranged loosely, while the sponge layer cells were relatively loose in structure with serious lignification. Additionally, the surface texture of plant leaves and young fruits exocarp was rough and wrinkled;the stomatal guard cells shrank, and the tissue around stomata shrank and appeared rough. Moreover, the glandular hairs on the epicarp surface of the susceptible fruit were raised and disordered. After virus infection, the sucrose content of No.1-3 leaves of susceptible plants was 23.11% higher than that of No. 4-6 leaves, while it remained relatively balanced in the healthy leaves. The starch accumulation, PG enzyme, cellulase and PPO enzyme activities were reduced;whereas, the activities of α-amylase, GS enzyme and POD enzyme in No.1-3 leaves of susceptible plants were increased. After being infected by TeMV and PWV, the distribution of nutrients in No.1-6 leaves of the plant was unbalanced. The synthesis and decomposition of related substances and antioxidant defense enzyme system were damaged, and the morphological structure of leaves and fruits were damaged, resulting in the disruption of the normal absorption function of the plant.

关 键 词：西番莲病毒 代谢生理 组织微结构 

分 类 号：S432.1[农业科学—植物病理学]