金属蛋白酶FocM352在香蕉枯萎病菌热带4号生理小种侵染过程中的功能  

Functional verification of M35 metalloprotease FocM352 in Fusarium oxysporum f. sp. cubense tropical race 4

作　　者：吴邦庭 刘思文 张晓霞 李春雨[2] 刘泓[1] WU Bangting;LIU Siwen;ZHANG Xiaoxia;LI Chunyu;LIU Hong(Fujian Agriculture and Forestry University,College of Resources and Environment,State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fuzhou 350002,China;Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization,Ministry of Agriculture,Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,China)

机构地区：[1]福建农林大学资源与环境学院,闽台作物有害生物生态防治国家重点实验室,福州350002 [2]广东省农业科学院果树研究所,农业部南亚热带果树生物学与遗传资源利用重点实验室,广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《植物病理学报》2023年第1期41-51,共11页Acta Phytopathologica Sinica

基　　金：广东省基础与应用基础研究基金-粤桂联合基金-重点项目(2020B1515420006)。

摘　　要：香蕉枯萎病菌热带4号生理小种(Fusarium oxysporum f. sp.cubense tropical race 4,Foc TR4)严重威胁世界香蕉产业的可持续发展,一些特殊功能的蛋白在其侵染过程中起重要作用。我们分析Foc TR4接种巴西蕉后的转录组数据发现M35金属蛋白酶(metalloprotease 35)同源基因FocM35_2在病原菌侵染初期受诱导显著上调表达,经同源重组法敲除该基因,突变体致病力显著下降,菌丝生长速率降低,产孢量略微减少,回补突变体则可以恢复其致病力和生长发育;与野生型菌株相比较,突变体对外源氧胁迫、渗透压和细胞膜压力更敏感;在本氏烟草(Nicotiana benthamiana)中的瞬时表达结果显示该蛋白定位于质外体,并引起叶片产生超敏反应(HR,hypersensitive response);该蛋白的缺失还能够诱导寄主更高水平几丁质酶的活性。因此,FocM35_2是促进Foc TR4侵染寄主的的重要致病因子。Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4(Foc TR4) is the most devastating disease on banana plants. In our previous study, FocM35_2 of Foc TR4 was identified from an earlier transcriptomics study on the infection process of banana roots. The FocM35_2 gene knockout mutant demonstrated a slightly vegetative growth defect, decreased sporulation rate and attenuated virulence. In addition, the mutant was more sensitive to exogenous oxygen stress, osmotic pressure and cell wall stress. Furthermore, transient expression of FocM35_2 in Nicotiana benthamiana showed that FocM35_2 could be expressed in the apoplastic region and induced HR in leaves. The absence of FocM35_2 also enhanced the enzymatic activity of chitinase in banana. The results showed that FocM35_2 is an important virulence factor in Foc TR4.

关 键 词：香蕉枯萎病菌 金属蛋白酶 基因敲除 细胞坏死 致病性 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]