水稻黑条矮缩病毒S6片段的序列多样性与进化分析  

Sequence diversity and evolutionary analysis of S6 segment of Rice black-streaked dwarf virus

作　　者：王馨玉 许振南 周羽[3] 翁建峰[2] 郭长虹[1] 李新海 WANG Xinyu;XU Zhennan;ZHOU Yu;WENG Jianfeng;GUO Changhong;LI Xinhai(College of Life Science and Technology,Haerbin Normal University,Haerbin150025,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing100081,China;College of Agriculture,Northeast Agricultu-ral University,Haerbin150030,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]中国农业科学院作物科学研究所,北京100081 [3]东北农业大学农学院,哈尔滨150030

出　　处：《植物病理学报》2023年第1期146-150,共5页Acta Phytopathologica Sinica

基　　金：中国博士后科学基金(2021M703553)。

摘　　要：0 引言植物RNA病毒在进化中具有快速变异的能力,有利于病毒扩大寄主范围甚至进化为新病毒[1]。突变和重组是植物RNA病毒变异和遗传多样性的共同进化因素,是群体基因变异的最初来源[2]。由灰飞虱传播的水稻黑条矮缩病毒(Rice black streaked dwarf virus, RBSDV) 属于呼肠孤病毒科(Reoviridae)斐济病毒属(Fijivirus),能引发玉米粗缩病和水稻黑条矮缩病。In order to deeply understand the variation and evolution of RBSDV S6 segment, we analyzed the genetic variation of RBSDV S6 sequence in 26 maize and rice samples from 13 locations. The results showed that there were a large number of base mutations in the coding region, indicating the presence of variation and recombination between S6 sequences. At the same time, by analyzing the amino acids and codon usage preferences encoded by S6, we found that S6 population has other codon usage preferences in addition to GC3s, and the codon usage of this population is under the pressure of natural selection. The genetic recombination analysis showed that there were 2 recombination events(S6-M-13-ⅤM-2 and S6-M-13-ⅤM-3) in 26 sequences. Further, based on phylogenetic analysis, S6 sequences can be divided into four groups. The above results show that S6 segment plays an important role in the genetic variation and evolution of RBSDV population, which provides a theoretical basis for further research on the pathogenic mechanism of RBSDV S6 in maize rough dwarf disease and rice black-streaked dwarf disease.

关 键 词：rice black-streaked dwarf virus silencing inhibitor P6 genetic variation evolutionary analysis 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]