不同浓度L-Met在拟南芥幼苗根系发育中的功能分析  

Functional Analysis of Different Concentrations of L-Met in Root Development of Arabidopsis thaliana Seedlings

作　　者：王雷 刘国兰 WANG Lei;LIU Guolan(College of Biological and Environmental Engineering,Binzhou University,Binzhou,Shandong 256603,China;Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta,Binzhou,Shandong 256603,China;Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta,Binzhou,Shandong 256603,China)

机构地区：[1]滨州学院生物与环境工程学院,山东滨州256603 [2]山东省黄河三角洲生态环境重点实验室,山东滨州256603 [3]山东省黄河三角洲野生植物资源开发利用工程技术研究中心,山东滨州256603

出　　处：《天津农业科学》2023年第2期11-15,共5页Tianjin Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2020QC038)。

摘　　要：为了解甲硫氨酸(Methionine, Met)在植物根系生长发育中的生理功能,以期为植物根系营养需求提供理论依据。通过采用不同浓度的L-Met(0、30、60、120μmol·L^(-1))处理拟南芥幼苗,分析7 d苗龄的主根生长发育情况,包括主根长度、分生组织长度生长情况。结果表明:低浓度L-Met(30μmol·L^(-1))促进主根生长,主根长,根分生组织长度及皮层细胞数目增大,而细胞大小并没有受到影响;高浓度(60μmol·L^(-1))及以上却抑制了根系发育,甚至导致分生组织分化,与对照相比形成显著性差异。综上,不同浓度L-Met处理拟南芥试验丰富了Met在植物根系生长发育的功能,为培育根系发达植物提供理论依据。In order to provide a theoretical basis for the nutritional requirements of plant roots, the physiological function of methionine in the growth and development of plant roots was studied. In this study, Arabidopsis thaliana seedlings were treated with different concentrations of L-Met(0, 30, 60, 120 μmol·L^(-1)) to analyze primary root growth and development at 7 days of seedling age, including primary root length and meristem length growth. The results showed that low concentration of L-Met(30 μmol·L^(-1)) promoted primary root growth, primary root length, root meristem length, and cortical cell number, but cell size was not affected. High concentrations(60μmol·L^(-1)) and above inhibited root development and even led to meristem differentiation, presenting a significant difference compared with the control. In conclusion, Arabidopsis thaliana treated with different concentrations of L-Met enriches the functions of Met in plant root growth and development, provides a theoretical basis for cultivating plants with developed roots.

关 键 词：甲硫氨酸 拟南芥 主根 生长发育 

分 类 号：Q945.32[生物学—植物学]