基于Wnt/β-catenin信号通路探讨川芎提取物对慢性心力衰竭大鼠的干预作用  

Intervention Effects of Ligusticum Chuanxiong Extract on Rats with Chronic Heart Failure Based on Wnt β-catenin Signaling Pathway

作　　者：高嘉 黄冀娜 李珍 GAO Jia;HUANG Jina;LI Zhen(Dept.of Cardiology,the Second Hospital of Baoding,Hebei Baoding 073000,China)

机构地区：[1]保定市第二医院心血管内科,河北保定073000

出　　处：《中国医院用药评价与分析》2023年第2期199-202,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：河北省医学科学研究课题计划(No.20200262)。

摘　　要：目的:探讨川芎提取物对慢性心力衰竭大鼠的干预作用,并通过Wnt/β-catenin信号通路探究其作用机制。方法:采用腹主动脉缩窄法结合竭力式游泳建立慢性心力衰竭大鼠动物模型,通过心电图检测大鼠造模前后心电图变化,建模成功后将大鼠分为模型组(n=10)和实验组(n=10),取10只未造模大鼠作为对照组。实验组大鼠给予川芎提取物(100 mg/kg),1日1次,连续干预4周;对照组和模型组大鼠分别给予等量0.9%氯化钠溶液灌胃。实验终点检测各组大鼠心率及呼吸频率,检测大鼠左心室舒张末期内径(LVIDd)、左心室收缩末期内径(LVIDs)、左心室射血分数(LVEF)和左心室缩短分数(LVFS)。采用苏木精-伊红(HE)染色检测大鼠心脏组织病理状态,采用电子显微镜检测大鼠心脏组织超微结构变化,采用蛋白质印迹法检测大鼠心脏组织Wnt/β-catenin信号通路关键分子去水平段极性蛋白3(Dvl3)、糖原合成酶激酶-3(GSKβ)和β-连环蛋白(β-catenin)表达情况。结果:超声检查结果表明,模型组大鼠的LVIDd、LVIDs显著高于对照组,LVEF、LVFS显著低于对照组;实验组大鼠的LVIDd、LVIDs显著低于模型组,LVEF、LVFS显著高于模型组,差异均有统计学意义(P<0.01)。HE染色结果和电镜结果表明,模型组大鼠心脏组织出现损伤,而实验组大鼠损伤小于模型组。蛋白质印迹法结果表明,模型组大鼠Dvl3、GSKβ和β-catenin表达水平显著高于实验组,而实验组相比于模型组有显著下降趋势,差异均有统计学意义(P<0.01)。结论:川芎提取物对慢性心力衰竭大鼠心脏有保护作用,其作用机制与Wnt/β-catanin信号通路的抑制有关。OBJECTIVE: To explore the intervention effects of Ligusticum Chuanxiong extract on rats with chronic heart failure and explore its mechanism through Wnt/β-catenin signaling pathway. METHODS: The rat model of chronic heart failure was established by abdominal aortic constriction combined with strenuous swimming. Electrocardiogram was used to detect the changes of electrocardiogram before and after modeling. After successful modeling, the rats were divided into the model group(n=10) and experimental group(n=10), and 10 rats without model were set as the control group. The experimental group was given Ligusticum Chuanxiong extract(100 mg/kg) once a day for 4 weeks, and the control group and model group received the same amount of 0.9% sodium chloride solution gavage. At the end of the experiment, the heart rate and respiratory rate of rats in each group were detected, and LVIDd, LVIDS, LVEF and LVFS were detected. The pathological state of rat heart tissue was detected by hematoxylin-eosin(HE) staining. Ultrastructural changes of heart tissues were detected by electron microscope, and the expression of key molecules Dvl3, GSKβ, β-catenin in heart tissues was detected by Western blotting. RESULTS: Ultrasound results showed that LVIDd and LVIDS of the model group were significantly higher than those of control group, LVEF and LVFS were significantly lower than those of control group, LVIDd and LVIDs of experimental group were significantly lower than those of model group, LVEF and LVFS were significantly higher than those of model group, the differences were statistically significant(P<0.01). HE staining and electron microscopy results showed that heart tissues of the model group were damaged, while the experimental group was less than the model group. Western blotting results showed that expression levels of Dvl3, GSKβ and β-catenin in the model group were significantly higher than those in the experimental group, and there was a significant downward trend compared with the model group(P<0.01). CONCLUSIONS: Ligu

关 键 词：川芎提取物 慢性心力衰竭 损伤 WNT/Β-CATENIN信号通路 

分 类 号：R932[医药卫生—生药学] R96[医药卫生—药学]