N^(4)-乙酰胞苷RNA检测技术的研究进展  

Research Advances of Detection Approaches Towards N^(4)-Acetylcytidine(ac^(4)C)RNA

作　　者：贺胤铭 孔素东 林建国 谢敏浩[2,3] 程靓 HE Yinming;KONG Sudong;LIN Jianguo;XIE Minhao;CHENG Liang(Beijing National Laboratory for Molecular Sciences(BNLMS),CAS Key Laboratory of Molecular Recognition and Function,CAS Research/Education Center for Excellence in Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China;School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China;NHC Key Laboratory of Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi 214063,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院化学研究所,北京分子科学国家研究中心,中国科学院分子科学科教融合卓越创新中心,分子识别与功能重点实验室,北京100190 [2]江南大学化学与材料工程学院,无锡214122 [3]国家卫生健康委员会核医学重点实验室,江苏省原子医学研究所,无锡214063 [4]中国科学院大学,北京100049

出　　处：《高等学校化学学报》2023年第3期167-174,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22022704,21977097)资助.

摘　　要：细胞中的mRNA和非编码RNA包含着大量的表观化学修饰.在这些修饰中,N^(4)-乙酰胞苷(ac^(4)C)是较为独特的一种,在真核生物和原核生物的tRNA,rRNA和mRNA中均有发现.研究表明,ac^(4)C RNA可能具有多种生物学功能,包括调节蛋白的翻译过程、影响RNA的稳定性及改变RNA-蛋白相互作用等.但当前对其修饰路径的研究还不成熟,催化ac^(4)C RNA形成的乙酰转移酶目前仅有NAT10被鉴定出来.不仅如此,目前对ac^(4)C RNA进行检测和测序的技术手段还相当局限.本文综合评述了ac^(4)C RNA的分布以及在基因表达调控中的作用和机制,重点介绍了ac^(4)C RNA的检测技术,并对ac^(4)C RNA当前研究中的不足和机遇进行了总结和展望.A large number of epigenetic chemical modifications have been detected in cellular mRNA and non-coding RNAs.Among these modifications,N^(4)-acetylcytidine(ac^(4)C)is a relatively unique one,which has widely been discovered in tRNA,rRNA and tRNAs in eukaryotes and prokaryotes.Current research have shown that ac^(4)C RNA may possess a variety of biological functions,including regulating the translation process of proteins,affecting the stability of RNA,and modulating RNA-protein interactions.However,only one ac^(4)C acetyltransferase,NAT10,has been identified so far.Besides,current technical methods for the detection and sequencing of ac^(4)C RNA are quite limited.This review comprehensively summarized current advances in the exploration of distribution of ac^(4)C RNA,its roles in gene expression regulation and its detection methods.Finally the opportunities and shortcomings of the related research on ac^(4)C were summarized and prospected.

关 键 词：N4-乙酰胞苷 RNA修饰 检测方法 

分 类 号：Q523[生物学—生物化学] Q752