植物生物反应器优化策略与最新应用  

Recent Advances in Optimization Strategies and Applications of Plant Bioreactors

作　　者：邹奇 潘炜松 邱健 束文圣 吴川 ZOU Qi;PAN Wei-song;QIU Jian;SHU Wen-sheng;WU Chuan(School of Metallurgy and Environment,Central South University,Changsha 410083,China;School of Life Sciences,South China Normal University,Guangzhou 510631,China;Hunan Novomore Biotechnology Corporation,Changsha 410016,China;Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]中南大学冶金与环境学院,长沙410083 [2]华南师范大学生命科学学院,广州510631 [3]湖南诺合新生物科技有限公司,长沙410016 [4]中南大学湘雅医院,长沙410008

出　　处：《中国生物工程杂志》2023年第1期71-86,共16页China Biotechnology

基　　金：湖南省自然科学基金(2021JJ30340);国家自然科学基金(31670955、42177392)资助项目。

摘　　要：随着分子生物学和植物基因工程的迅猛发展以及分子医药和现代农业等学科的交叉融合,植物生物反应器已成为分子医药农业的核心内容。利用植物生物反应器生产抗体、疫苗和功能性食品,具有规模化、成本低、安全性高、周期短等优势。2022年2月,加拿大卫生部批准了新型冠状病毒疫苗Covifenz?,这是世界首款植物源人体疫苗,标志着以植物生物反应器为代表的分子医药农业时代的来临。综述植物叶片和种子等代表性的植物生物反应器类型,分析瞬时表达系统和稳定表达系统的构建原理和应用,探讨通过启动子和密码子优化、糖基化过程“人源化”、基因沉默抑制和蛋白酶作用抑制等优化植物生物反应器的策略,总结国内外抗体、疫苗和功能性食品等植物源产品的开发进展,以期为我国植物生物反应器的研究及其在分子医药领域的应用提供参考。Plant bioreactors have been the central part of molecular pharming. Vaccines, antibodies and functional foods produced by plant bioreactors with the benefits of cost-effectiveness, high scalability, rapid production, enabling post-translational modification, and no harmful pathogens contamination are increasingly accepted by the public. In February 2022, Health Canada approved the world’s first plant-derived human vaccine Covifenz? for the prevention and treatment of COVID-19, marking the advent of the era of molecular pharming represented by plant bioreactors. This paper elaborates the development history of plant bioreactors, with the main host species representatives of leafy plants and seed plants, the stable and transient expression systems construction for various applications, as well as the enhancement strategies through promoter and codon optimization, “humanization” of glycosylation process, inhibition of gene silencing and protease activity, and also summarize the application of plant-derived protein products, which aim to provide a theoretical and application basis for the development of plant bioreactors.

关 键 词：植物生物反应器 分子医药农业 外源蛋白 瞬时和稳定表达 

分 类 号：Q819[生物学—生物工程]