烟草glu-CMS不育系细胞学和转录组分析  

Cytological and transcriptome analysis of cytoplasmic male sterility and maintainer line in glu-CMS tobacco

作　　者：陈慧[1] 钱保 裴肖雄 李艺凡 黄昌军[2] 刘勇[2] 袁诚 于海芹[2] 曾建敏[2] 易斌[1] CHEN Hui;QIAN Bao;PEI Xiaoxiong;LI Yifan;HUANG Changjun;LIU Yong;YUAN Cheng;YU Haiqin;ZENG Jianmin;YI Bin(Huazhong Agricultural University,Wuhan 430000,Hubei,China;Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650000,Yunan,China)

机构地区：[1]华中农业大学,湖北武汉430000 [2]云南省烟草农业科学研究院,云南昆明650000

出　　处：《中国烟草学报》2023年第1期64-70,共7页Acta Tabacaria Sinica

基　　金：中国烟草总公司科技项目“烟草野火病抗性基因定位及K326品种抗性改良育种研究”(NO.110202101044(JY-21));中国烟草总公司云南省公司科技项目“烟草雄性不育胞质Nta(gla.)S的效应评价及利用研究”(NO.2019530000241004);“烟草野火病抗性基因定位及K326品种抗性改良育种研究”(NO.2022530000241010);“烟草雄性不育核基因功能鉴定及不育系材料创制”(2022530401641004)。

摘　　要：【背景和目的】单一胞质在烟草杂交种的大量运用导致生产用种遗传基础脆弱,为拓宽不育胞质种类,对glu-CMS的不育机制进行了探究。【方法】首先对glu-CMS不育系表型进行考察,随后对glu-CMS不育系和其保持系0.75 mm和1.5 mm的花蕾进行转录组测序分析及GO和KEGG富集分析。【结果】(1)glu-CMS不育系表现出雄蕊缺失和雄蕊发育异常等现象。(2)共鉴定出2075个差异基因,主要富集在植物信号转导、碳水化合物代谢和ROS调控通路。【结论】本研究揭示了glu-CMS不育系的表现形式以及不育系和保持系的差异表达基因,为glu-CMS的后续研究提供一定理论基础。[Background and Objective] The large application of single cytoplasm in tobacco hybrids has resulted in the fragile genetic basis of cultivar. In order to broaden the variety of sterile cytoplasm, the sterility mechanism of glu-CMS was investigated in this study.[Methods] Firstly, the phenotypes of glu-CMS male sterile lines were investigated, and then the flower buds of glu-CMS male sterile lines and their maintainers at 0.75 mm and 1.5 mm were analyzed by transcriptomic sequencing and GO and KEGG enrichment analysis.[Results](1) Stamen deletion and abnormal stamen development were observed in glu-CMS CMS lines.(2) A total of 2075 differential genes were identified, which were mainly enriched in the pathways for plant signal transduction, carbohydrate metabolism and ROS regulation. [Conclusion] This study reveals the expression forms of glu-CMS and the differential gene expression between CMS and maintainer lines, providing a theoretical basis for the subsequent research of glu-CMS.

关 键 词：烟草 glu-CMS 转录组分析 

分 类 号：S572[农业科学—烟草工业]