淫羊藿苷对膝创伤性关节炎模型大鼠的镇痛效果及机制  

Analgesic effect and mechanism of icariin in a rat model of post-traumatic knee arthritis

作　　者：丰瑞兵 黄勇 胡昊 吴刚[1,2] 段小锋 李朝文 Feng Ruibing;Huang Yong;Hu Hao;Wu Gang;Duan Xiaofeng;Li Chaowen(Department of Spine Surgery,Hubei Hospital of TCM,Wuhan 430074,Hubei Province,China;School of Health Sciences,Wuhan Sports University,Wuhan 430079,Hubei Province,China)

机构地区：[1]湖北省中医院脊柱外科,湖北省武汉市430074 [2]武汉体育学院健康科学学院,湖北省武汉市430079

出　　处：《中国组织工程研究》2023年第32期5108-5113,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:创伤性关节炎是骨关节炎最常见的形式之一,体内外研究显示,淫羊藿苷能通过多靶点、多途径、多通路对骨关节炎起到治疗作用,并且无明显不良反应,但淫羊藿苷对创伤性关节炎的镇痛效果及作用机制尚未见报道。目的:探讨淫羊藿苷对大鼠膝创伤性关节炎的镇痛效果及机制。方法:30只雄性SD大鼠随机分为假手术组、模型组和淫羊藿苷组,每组10只。后两组切断大鼠右膝前交叉韧带造模,假手术组仅切开右膝关节囊。造模结束后,淫羊藿苷组大鼠给予淫羊藿苷溶液100 mg/(kg·d)灌胃,假手术组与模型组同时期给予等量生理盐水灌胃,连续8周。给药结束后,采用机械刺激法与热辐射法检测机械撤退阈值与热撤退潜伏期,以此评估大鼠的疼痛阈值;应用旷场实验评估大鼠焦虑、抑郁状态;对膝关节软骨组织进行大体观察及Pelletier评分;以番红固绿染色与国际骨关节炎研究学会评分评估软骨退变程度;采用ELISA检测关节液与滑膜组织炎症因子白细胞介素1β、肿瘤坏死因子α的质量浓度;蛋白印迹法检测软骨组织单核细胞趋化蛋白1/单核细胞膜受体2配体受体轴的蛋白表达水平。结果与结论:①与模型组相比,淫羊藿苷组的机械撤退阈值与热撤退潜伏期均显著升高(P<0.05);②与假手术组相比,模型组大鼠运动总距离、总穿格数、中央区滞留时间均显著减少(P<0.05),与模型组相比,淫羊藿苷组大鼠上述3个指标均显著增加(P<0.05);③与模型组相比,淫羊藿苷组膝关节液及滑膜组织中白细胞介素1β、肿瘤坏死因子α的质量浓度均显著降低(P<0.05);④与假手术组相比,模型组关节软骨面多表现为凹凸不平、形变,软骨组织肥大、硬化无光泽,大量软骨组织剥脱;而淫羊藿苷组较模型组软骨退变明显减轻;⑤番红固绿染色与国际骨关节炎研究学会评分结果显示,与假手术组相比,模型组膝关节BACKGROUND:Post-traumatic arthritis is one of the most common forms of osteoarthritis.In vivo and in vitro studies have shown that icariin can treat osteoarthritis through multiple targets and multiple pathways with no obvious side effects.However,the analgesic effect and mechanism of icariin on posttraumatic arthritis has not been reported.OBJECTIVE:To investigate the analgesic effect and mechanism of icariin on post-traumatic arthritis in rats.METHODS:Thirty male Sprague-Dawley rats were randomly divided into sham group(n=10),model group(n=10)and icariin group(n=10).The right knee anterior cruciate ligament was cut off in the model group and icariin group,while the right knee joint capsule was cut only in the sham group.The rats in the icariin group were given intragastric administration of icariin(100 mg/kg/d),and the sham and model groups were given the same amount of normal saline at the same time point for 8 weeks.Mechanical withdrawal threshold and thermal withdrawal latency were measured by mechanical stimulation and thermal radiation to evaluate the pain threshold of rats.Open field test was used to evaluate anxiety and depression.General observation and Pelletier scoring of the knee cartilage were performed.Safranin O-Fast green staining and Osteoarthritis Research Society International score were used to evaluate the degree of cartilage degeneration.ELISA was used to detect the contents of inflammatory factors interleukin-1βand tumor necrosis factor-αin synovial fluid and synovial tissue.The protein expression of monocyte chemotactic protein 1/chemokine C-C-motif receptor 2 ligand receptor axis in cartilage tissue was detected by western blot.RESULTS AND CONCLUSION:The mechanical withdrawal threshold and thermal withdrawal latency of the icariin group were significantly higher than those of the model group(P<0.05).Compared with the sham group,the total exercise distance,the total number of crossing squares and the residence time in the central area of the model group were significantly decreased(P<0.

关 键 词：淫羊藿 创伤性关节炎 疼痛 炎症因子 单核细胞趋化蛋白1 单核细胞膜受体2 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R684.3