离心运动模式对运动性肌肉损伤的影响  

Effects of eccentric exercise patterns on exercise-induced muscle damage

作　　者：孟祥利 吕晓虹 Meng Xiangli;Lyu Xiaohong(Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China;Department of Radiology,First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China)

机构地区：[1]锦州医科大学,辽宁省锦州市121000 [2]锦州医科大学附属第一医院放射科,辽宁省锦州市121000

出　　处：《中国组织工程研究》2023年第32期5132-5136,共5页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省教育厅2020年度科学研究项目(JYTJCZR2020058),项目负责人:吕晓虹;辽宁省2021年度大学生创新创业训练计划项目(S202110160025),项目负责人:孟祥利。

摘　　要：背景:运动性肌肉损伤在各种运动损伤类型中所占的比率最高,离心运动模式可能与其损伤程度相关,怎样有效利用骨骼肌离心收缩对全民健身和体育运动事业的发展具有重要意义。目的:探讨不同的离心运动方式对运动性肌肉损伤程度的影响。方法:将66只SD雄性大鼠随机分为对照组(n=6)、一次离心运动组(n=30)、持续离心运动组(n=30)。按照最后1次运动后0.5,24,48,120,168 h分为5个时间亚组,取股四头肌组织后使用免疫组化染色观察白细胞介素6、肿瘤坏死因子α的表达并检测平均吸光度值,苏木精-伊红染色观察肌纤维形态变化。结果与结论:①对照组白细胞介素6、肿瘤坏死因子α轻度细胞质棕染;运动组在0.5 h即出现白细胞介素6、肿瘤坏死因子α表达增加;24-48 h肌纤维扭曲、排列及轮廓紊乱,细胞外间隙伴随炎细胞浸润增大,胞质棕色染色程度及范围增加至峰值;120,168 h胞质棕色染色程度及范围表达降低;②在48,120,168 h,持续离心运动组白细胞介素6、肿瘤坏死因子α的平均吸光度均值低于一次离心运动组(P<0.05);③持续离心运动组、一次离心运动组苏木精-伊红染色的骨骼肌形态结构恢复时间不同,分别为120 h和168 h;④双因素重复测量方差分析结果显示运动模式和时间点与白细胞介素6、肿瘤坏死因子α的平均吸光度值均有统计学意义,并且模式和时间存在交互作用,F值分别为3.106,13.431(P=0.020,P<0.001);⑤结果表明,运动性肌肉损伤的程度与离心运动模式相关,持续离心运动优于一次离心运动。BACKGROUND:Exercise-induced muscle damage accounts for the highest proportion among various sports injury types,and eccentric exercise patterns may be related to the severity of damage.An effective utilization of skeletal muscle eccentric contraction is of great significance to the development of national fitness and sports undertakings in line with the concept of"healthy China."OBJECTIVE:To investigate the influence of different eccentric exercise patterns on the degree of exercise-induced muscle damage.METHODS:A total of 66 male Sprague-Dawley rats were randomly divided into control group(n=6),single eccentric exercise group(n=30),and continuous eccentric exercise group(n=30)which was further divided into five subgroups at 0.5,24,48,120,168 hours after the last exercise.The expressions of interleukin-6 and tumor necrosis factor-αin the quadriceps femoris were detected by immunohistochemical staining,and the mean absorbance value was measured.Hematoxylin-eosin staining was used to observe the morphological changes of muscle fibers.RESULTS AND CONCLUSION:There were cells with mild cytoplasmic brown staining of interleukin-6 and tumor necrosis factor-αin the control group.In the exercise groups,the expression of interleukin-6 and tumor necrosis factor-αbegan to increase at 0.5 hours.From 24 to 48 hours,muscle fibers were distorted with disordered arrangement and contours,the extracellular space was enlarged with inflammatory cell infiltration,the degree and range of cytoplasmic brown staining increased to the peak but it decreased at 120 and 168 hours.The mean absorbance values of interleukin-6 and tumor necrosis factor-αin the continuous eccentric exercise group were lower than those in the once eccentric exercise group at 48,120,and 168 hours(P<0.05).The recovery time of morphological structure of the skeletal muscle stained by hematoxylin-eosin was different in the continuous eccentric exercise group and once eccentric exercise group,which was 120 and 168 hours respectively.Results from the two-way repeated

关 键 词：运动性肌肉损伤 肌肉酸痛 离心运动 骨骼肌 影响因素 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R722.142