柚皮苷对骨质疏松大鼠拔牙创骨愈合的影响  

Effects of naringin on healing of tooth extraction wound in osteoporotic rats

作　　者：黄浩哲 郑敏 李娜 孙黎波 兰玉燕 刘敏 Huang Haozhe;Zheng Min;Li Na;Sun Libo;Lan Yuyan;Liu Min(Department of Prosthodontics,The Affiliated Stomatological Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Luzhou Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,Luzhou 646000,Sichuan Province,China;Institute of Stomatology,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Stomatology,Luzhou People’s Hospital,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属口腔医院口腔修复科,四川省泸州市646000 [2]口颌面修复重建和再生泸州市重点实验室,四川省泸州市646000 [3]西南医科大学口腔医学研究所,四川省泸州市646000 [4]四川省泸州市人民医院口腔科,四川省泸州市646000

出　　处：《中国组织工程研究》2023年第32期5144-5149,共6页Chinese Journal of Tissue Engineering Research

基　　金：四川省泸州市科技局[2017-S-42(1/2)],项目负责人:孙黎波;四川省大学生创新创业训练计划项目(Q18011),指导教师:兰玉燕;四川省大学生创新创业训练计划项目(S202210632150),指导教师:兰玉燕。

摘　　要：背景:柚皮苷作为一种中药单体化合物,具有抗炎、促成骨的作用,但其对拔牙创愈合速度的影响并不明确。目的:建立骨质疏松大鼠拔牙模型,通过体内实验探究柚皮苷对拔牙创愈合速度的影响。方法:48只雌性SD大鼠随机分为假手术组14只与去势组34只。去势组去除双侧卵巢建立骨质疏松模型,6周后两组各随机取4只大鼠行MicroCT检测确认建模结果。建模成功后将剩余大鼠随机分为对照组、柚皮苷及雌二醇组,均拔除左上颌第二磨牙,柚皮苷组予以300 mg/(kg·d)柚皮苷灌胃,雌二醇组予以20μg/(kg·d)17β雌二醇皮下注射,对照组予以等体积生理盐水处理。干预4周后,分离各组大鼠上颌骨进行苏木精-伊红染色、Masson染色、MicroCT检测,检测拔牙创周围骨组织骨保护素、核因子κB受体活化因子配体(receptor activator of nuclear factor-κB ligand,RANKL)mRNA及蛋白表达量。结果与结论:①去势6周后,去势组骨体积分数降低,证明骨质疏松建模成功;②拔牙4周后,与对照组相比,柚皮苷组、雌二醇组骨小梁间距小、骨小梁数量多,牙槽骨高度丢失更少,说明柚皮苷可促进拔牙窝骨愈合;③与对照组相比,柚皮苷组、雌二醇组拔牙创周围骨组织骨保护素表达升高、RANKL表达降低,说明柚皮苷可以通过上调骨保护素和下调RANKL的表达水平,促进骨质疏松大鼠拔牙创骨愈合。BACKGROUND:Naringin,a monomer compound of Traditional Chinese medicine,has anti-inflammatory and osteogenic effects,but its effect on the healing rate of tooth extraction wounds is not clear.OBJECTIVE:To investigate the effect of naringin on the healing rate of tooth extraction wounds in rat models of osteoporosis.METHODS:Forty-eight female Sprague-Dawley rats were randomly divided into sham-operation group(n=14)and ovariectomized group(n=34).In the ovariectomized group,bilateral ovaries were removed to establish osteoporosis models.After 6 weeks,four rats in each group were randomly selected for MicroCT detection to confirm the modeling results.After successful modeling,the remaining rats were subject to tooth extraction and randomly divided into tooth extraction group(the remaining rats in the sham-operation group),control group,naringin group,and estradiol group,and the second molar of left maxillary was extracted.Naringin group was given naringin 300 mg/kg/d by gavage,estradiol group was given 17-βestradiol 20μg/kg/d by subcutaneous injection,and tooth extraction group and control group were treated with the same volume of normal saline.After 4 weeks of intervention,the rat maxilla was separated for hematoxylin-eosin staining,Masson staining,and MicroCT detection.The expressions of osteoprotegerin and receptor activator of nuclear factor-κB ligand(RANKL)at mRNA and protein levels in the bone tissue around the tooth extraction wounds were also detected.RESULTS AND CONCLUSION:After 6 weeks of castration,the bone volume fraction of the ovariectomized group decreased,indicating that the modeling of osteoporosis was successful.Four weeks after tooth extraction,compared with the control group,the naringin group and estradiol group had smaller trabecular separation and less loss of alveolar bone height,indicating that naringin could promote bone healing of tooth extraction wounds.Compared with the control group,the expression level of osteoprotegerin and RANKL in bone tissues around tooth extraction was increased

关 键 词：骨质疏松症 柚皮苷 拔牙创 骨愈合 OPG RANKL RANK 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R782.11