热敏灸银屑病模型大鼠外关穴的生物学变化  

Biological changes of Waiguan acupoint in a rat model of psoriasis undergoing heat-sensitive moxibustion

作　　者：陈楚 欧阳厚淦[1] 朱绪映 齐艳喆 王姿 Chen Chu;Ouyang Hougan;Zhu Xuying;Qi Yanzhe;Wang Zi(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi Province,China)

机构地区：[1]江西中医药大学,江西省南昌市330004

出　　处：《中国组织工程研究》2023年第32期5150-5154,共5页Chinese Journal of Tissue Engineering Research

基　　金：江西省自然科学基金面上项目(20202BABL206143),项目负责人:欧阳厚淦;江西省教育厅科技项目重点课题(GJJ201213),项目负责人:欧阳厚淦。

摘　　要：背景:在临床治疗以及基础研究中热敏灸运用广泛,但其敏化穴位的生物学特性尚未完全明确。目的:探讨热敏灸对银屑病模型大鼠结缔组织、钙离子以及太赫兹波的影响以及相互之间的关系。方法:采用5%咪喹莫特乳膏诱导银屑病SD大鼠模型,将实验大鼠随机分为空白组、模型组、治疗组。除空白组外,其余各组大鼠背部和手少阳三焦经循行路线处皮肤进行银屑病皮损造模,造模成功后治疗组于每日同一时间对手少阳三焦经外关穴进行灸疗,每次40 min,连续7 d,在治疗期间尾温波动>1℃为热敏灸组,尾温波动≤1℃为艾灸组。苏木精-伊红染色观察大鼠皮下结缔组织的病理学变化,比色法检测皮下筋膜钙离子变化,太赫兹波探测仪检测外关穴的太赫兹波电压均值变化。结果与结论:①与空白组比较,模型组大鼠结缔组织病理学改变明显;②与空白组和模型组相比,艾灸组和热敏灸组皮下筋膜钙离子浓度增高(P<0.01);与艾灸组相比,热敏灸组皮下筋膜钙离子浓度明显增高(P<0.01);③治疗组在治疗第3天时,太赫兹波电压均值高于模型组和空白组(P<0.01),第7天时,艾灸组的太赫兹波电压均值仍高于模型组和空白组(P<0.01),而热敏灸组的太赫兹波电压均值则接近于空白组;与艾灸组相比,热敏灸组治疗第3天的太赫兹波电压均值明显增高(P<0.01),第7天时则明显下降(P<0.01);④结果表明,在热敏灸治疗过程中银屑病模型大鼠穴位局部的钙离子出现富集现象,太赫兹波也有改变,热敏灸、钙离子以及太赫兹波之间或存在密切联系,从而治疗或改善银屑病症状。BACKGROUND: Heat-sensitive moxibustion is widely used in clinical treatment and basic research, but the biological characteristics of its sensitized acupoints are not completely clear.OBJECTIVE: To explore the effects of heat-sensitive moxibustion on connective tissue, calcium and terahertz wave in psoriatic rats and the relationship between them.METHODS: Animal models of psoriasis were induced by 5% imiquimod cream in Sprague-Dawley rats, and the experimental rats were randomly divided into blank group, model group and treatment groups. Except the blank group, the rats in the other groups were modeled with psoriatic lesions on the back and along the Sanjiao meridian of hand-Shaoyang. After successful modeling, the treatment group was treated with moxibustion at Waiguan acupoint at the same time every day, 40 minutes once for 7 days in total. During the treatment period, the tail temperature changed > 1 ℃ was considered to be the heat-sensitive moxibustion group, and the tail temperature changed ≤ 1 ℃ was considered to be the moxibustion group. Hematoxylin-eosin staining was used to observe the pathological changes of subcutaneous connective tissue, colorimetric method was used to detect the changes of calcium ions in subcutaneous fascia, and terahertz wave detector was used to detect the changes of mean terahertz wave voltage at Waiguan point.RESULTS AND CONCLUSION: Compared with the blank group, the pathological changes of connective tissue were obvious in the model group. Compared with the blank group and model group, the calcium ion concentration in subcutaneous fascia was significantly higher in the moxibustion group and heat-sensitive moxibustion group(P < 0.01). The calcium ion concentration in the subcutaneous fascia was significantly higher in the heat-sensitive moxibustion group than the moxibustion group(P < 0.01). On the 3rdday of treatment, the mean terahertz wave voltage values in the treatment groups were higher than those in the model group and the blank group(P < 0.01). On the 7thday, the m

关 键 词：热敏灸 银屑病 结缔组织 钙离子 太赫兹波 模型大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R275.9