改良椎板切除法构建脊髓损伤模型大鼠  

Improved laminectomy for constructing a rat model of spinal cord injury

作　　者：杜凯然 邓强[2] 郭铁峰[2] 张彦军[2] 彭冉东 李军杰 王雨榕 张凯东 罗林钊 Du Kairan;Deng Qiang;Guo Tiefeng;Zhang Yanjun;Peng Randong;Li Junjie;Wang Yurong;Zhang Kaidong;Luo Linzhao(Gansu University of Chinese Medicine,Lanzhou 730030,Gansu Province,China;Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu Province,China)

机构地区：[1]甘肃中医药大学,甘肃省兰州市730030 [2]甘肃省中医院,甘肃省兰州市730050

出　　处：《中国组织工程研究》2023年第32期5173-5177,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(82060879),项目负责人:邓强;国家自然科学基金(81860860),项目负责人:邓强;甘肃省重点研发计划(20YF3FA014),项目负责人:邓强。

摘　　要：背景:目前脊髓损伤已成为世界性难题,实验动物造模是探索疾病的第一步,但现缺乏较为有效的实验动物模型。目的:建立一种可复制、可调控、创伤小、死亡率低、出血量少、适用范围广、术中时间短的脊髓损伤大鼠模型。方法:将选用的体质量相似的40只SD大鼠随机分为常规组和改进组,每组20只,常规组采用Allen法构建脊髓损伤模型,改良组在原有模型的基础上,运用牙科磨钻代替器械咬除,比较两组大鼠手术时间、术中出血量、死亡率及击打后1,3,6,9,12,15 d的BBB运动功能学评分。结果与结论:改良继发性脊髓损伤模型建立方法比常规建模方法出血量少、死亡率低、造模时间短、BBB评分结果更集中,由此可证明改良后的击打方法更适用于继发性脊髓损伤模型的建立。BACKGROUND:Spinal cord injury has become a worldwide problem.Experimental animal modeling is the first step to explore the disease;however,there is a lack of experimental animal models that are more effective.OBJECTIVE:To establish a rat model of spinal cord injury that can be replicated,regulated,has little trauma,low mortality,low blood loss,wide application and short intraoperative time.METHODS:Forty Sprague-Dawley rats with similar body mass were selected and randomly divided into conventional model group and improved model group(n=20 per group),where the model of spinal cord injury was made using Allen’s method and improved method,respectively.In the improved model group,a dental drill was used for biting.Operation time,intraoperative blood loss,mortality,and Basso-Beattie-Bresnahan locomotor rating scale scores at 1,3,6,9,12,and 15 days after beating were compared between the two groups.RESULTS AND CONCLUSION:The improved method for constructing the animal model of secondary spinal cord injury has the advantages of shorter modeling time,less intraoperative blood loss,lower mortablity,and more centralized Basso-Beattie-Bresnahan score data as compared with the conventional method,which proves that the improved method is more suitable for establishing the model of secondary spinal cord injury.

关 键 词：脊髓损伤 动物模型 Allen法 改良 大鼠 运动功能 BBB评分 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R744