2型糖尿病小鼠牙齿牙合向伸长运动过程中根尖周组织的变化  

Changes in periapical tissue of type 2 diabetic mice during tooth occlusal elongation

作　　者：郑毅[1] 魏舟丹 郭栋 师子清 牛蔚然 许倬瑜 李鹏翠[6] 李文晋[3] Zheng Yi;Wei Zhoudan;Guo Dong;Shi Ziqing;Niu Weiran;Xu Zhuoyu;Li Pengcui;Li Wenjin(Stomatology Hospital of Tianjin Medical University,Tianjin 300070,China;Department of Stomatology,Putuo District People's Hospital of Shanghai,Shanghai 200333,China;Department of Stomatology,The Second Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi Province,China;Shanxi Medical University,Taiyuan 030001,Shanxi Province,China;Shanda University,Shanghai 201209,China;Department of Orthopedics,The Second Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi Province,China)

机构地区：[1]天津医科大学口腔医院,天津市300070 [2]上海市普陀区人民医院口腔科,上海市200333 [3]山西医科大学第二医院口腔科,山西省太原市030001 [4]山西医科大学,山西省太原市030001 [5]上海杉达学院,上海市201209 [6]山西医科大学第二医院骨科,山西省太原市030001

出　　处：《中国组织工程研究》2023年第32期5196-5202,共7页Chinese Journal of Tissue Engineering Research

基　　金：山西省卫生健康委科研课题(2019042),项目负责人:李文晋;山西省留学人员科技活动择优资助项目(20200010),项目负责人:李文晋。

摘　　要：背景:临床工作中经常会遇到一些糖尿病患者的牙齿缺失情况,牙齿缺失后如果未及时行修复治疗,会导致对颌牙伸长,引起垂直向牙合间隙减少、咬合早接触、牙合干扰,甚至需要拔除等一系列问题。目的:观察糖尿病小鼠牙齿牙合向伸长过程中根尖周组织内晚期糖基化终产物受体和骨唾液蛋白的表达,探究糖尿病在牙齿牙合向伸长运动过程中对根尖周组织的影响。方法:将120只雄性C57BL小鼠随机分为3组,即糖尿病组、阴性对照组和空白对照组(n=40)。糖尿病组腹腔注射70 mg/kg的链脲佐菌素建立糖尿病模型,阴性对照组腹腔注射等量的柠檬酸钠溶液,空白对照组则不做处理。建模成功后,3组中均随机选出30只小鼠拔除右侧上颌磨牙,再将3组小鼠分为0,3,6,9,12 d 5个亚组(n=6),按组别定时处死,提取右侧下颌骨。通过实时荧光定量PCR及免疫组化染色观察小鼠磨牙根尖周组织中晚期糖基化终产物受体和骨唾液蛋白的mRNA和蛋白表达水平。结果与结论:①实时荧光定量PCR结果:晚期糖基化终产物受体mRNA相对表达量糖尿病组高于其他两组,骨唾液蛋白mRNA的相对表达量糖尿病组少于其他两组(P<0.05),阴性对照组与空白对照组相比无明显差异(P>0.05);随着拔牙时间延长,3组中2种基因的相对表达量均呈持续增长的趋势,糖尿病组在3-6 d时表达量增加最快,阴性对照组及空白对照组在6-9 d时增长速度较快;糖尿病组晚期糖基化终产物受体mRNA相对表达量的增长趋势整体较其他两组明显,骨唾液蛋白mRNA相对表达量的增长趋势整体较其他两组平缓;②免疫组化染色结果:3组小鼠磨牙根尖周晚期糖基化终产物受体和骨唾液蛋白的阳性率随拔牙时间延长均越来越高;糖尿病组晚期糖基化终产物受体阳性率在拔牙后各时间点均显著高于其他两组(P<0.05);骨唾液蛋白阳性率在拔牙后的6,9,12 d显著低于其他BACKGROUND:In clinical work,some diabetic patients often have tooth loss.If the tooth loss is not timely repaired,it will lead to the elongation of the maxillary teeth,resulting in the reduction of the vertical occlusal gap,early occlusal contact,occlusal interference,and even tooth extraction.OBJECTIVE:To observe the expression of receptor for advanced glycation end products(RAGE)and bone sialoprotein(BSP)in periapical tissue of diabetic mice during tooth occlusal elongation,and explore the effect of diabetes on periapical tissues.METHODS:Totally 120 male C57BL mice were divided randomly into diabetic group(n=40),negative control group(n=40)and blank control group(n=40).Animal models of type II diabetes were established in the diabetic group by intraperitoneal injection of 70 mg/kg streptozotocin.The negative control group was intraperitoneally injected with the same amount of sodium citrate solution,while the blank control group was not disposed.After modeling,30 mice randomly were selected from each group to extract the right maxillary molars.Then,the mice were further divided into five subgroups of 0,3,6,9 and 12 days(n=6 per group).The mice were killed respectively according to the time specified by each group to obtain their right mandibles.The mRNA and protein expression levels of RAGE and BSP in periapical tissue were detected by real-time PCR and immunohistochemistry,respectively.RESULTS AND CONCLUSION:Real-time PCR results indicated significantly higher RAGE and lower BSP relative mRNA expression levels in the diabetic group than the blank and negative control groups(P<0.05),while there was no significant difference between blank and negative control groups(P>0.05).With the time extension of tooth extraction,the relative expression levels of two genes in the three groups both showed a trend of continuous growth.In the diabetic group,the expression levels increased obviously from 3 to 6 days,while in the blank and negative control groups,the expression levels raised apparently from 6 to 9 days.The increa

关 键 词：骨唾液蛋白 糖尿病小鼠 晚期糖基化终产物受体 牙齿伸长 免疫组化染色 实时荧光定量PCR 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1