儿童肺炎支原体肺炎IL-6/STAT3信号通路对肺功能及Th17/Treg的影响  

Impact of IL-6/STAT3 signaling pathways on lung function and Th17/Treg of children with Mycoplasma pneumoniae pneumonia

作　　者：李会娜[1] 郝恒瑞 安红[1] 马红芬 刘磊 LI Hui-na;HAO Heng-rui;AN Hong;MA Hong-feng;LIU lei(Xingtai People′s Hospital,Xingtai,Hebei 054000,China;不详)

机构地区：[1]邢台市人民医院儿科,河北邢台054001 [2]石家庄金域医学检验实验室有限公司,河北石家庄050035

出　　处：《中华医院感染学杂志》2023年第3期420-423,共4页Chinese Journal of Nosocomiology

基　　金：河北省卫生厅重点科技研究计划基金资助项目(20211064)。

摘　　要：目的 探讨儿童肺炎支原体肺炎白细胞介素-6(IL-6)/信号转导子和转录激活子3(STAT3)信号通路与肺功能及Th17/Treg。方法 选取2021年5月-2022年2月邢台市人民医院接受治疗的150例肺炎支原体肺炎患儿作为研究组,随机纳入同期于医院体检的儿童50名作为对照组。检测白细胞介素-6(IL-6)、C-反应蛋白(CRP)、降钙素原(PCT)水平;流式细胞术检测Th17、Treg,计算Th17/Treg;采用实时荧光定量聚合酶链式反应(PCR)检测STAT3 mRNA的表达;检测两组的肺功能指标:第1秒用力呼气容积(FEV_(1))、呼气峰值流速(PEF)及FEV_(1)/用力肺活量(FVC)。结果 研究组患儿IL-6、PCT和CRP均高于对照组(P<0.001);研究组患儿Th17及Th17/Treg高于对照组,Treg低于对照组(P<0.001);研究组患儿STAT3 mRNA高于对照组(P<0.05);对照组患儿PEF、FEV_(1)%以及FEV_(1)/FVC肺功能指标优于研究组患儿(P<0.001)。结论 肺炎支原体肺炎患儿体内Th17/Treg细胞失衡,IL-6/STAT3信号通路相关分子水平升高,导致患儿肺功能下降,推测IL-6/STAT3信号通路的激活可能引发了Th17/Treg细胞失衡,进而影响相应细胞因子水平,导致了肺炎支原体肺炎发生与发展。OBJECTIVE To investigate the role of IL-6/STAT3 signaling pathway in lung function and Th17/Treg cells imbalance in children with Mycoplasma pneumonia.METHODS From May 2021 to Feb.2022,150 children with Mycoplasma pneumonia who were treated in Xingtai People’s Hospital were selected as the study group,and 50 children who came to our hospital for physical examination during the same period were randomly included as the control group.ELISA was used to detect the levels of interleukin-6(IL-6),C-reactive protein(CRP),and procalcitonin(PCT);flow cytometry was used to detect Th17 and Treg,and Th17/Treg was calculated;real-time fluorescence quantitative PCR was used to detect STAT3 mRNA expression;pulmonary function indexes of the two groups were detected,such as forced expiratory volume in 1 second(FEV_(1)),peak expiratory flow rate(PEF) and 1 second rate(FEV_(1)/FVC).RESULTS Compared with the control group,IL-6,PCT and CRP in the study group were significantly increased(P<0.001);the Th17 percentage and the Th17/Treg ratio in the study group were significantly increased,and the Treg percentage was significantly decreased.STAT3 mRNA in the study group was significantly higher than that in the control group(P<0.001);the PEF,FEV_(1)% and FEV_(1)/FVC pulmonary function indexes of the children in the control group were significantly better than those in the study group(P<0.001).CONCLUSION The imbalance of Th17/Treg cells in children with Mycoplasma pneumonia and the increase of IL-6/STAT3 signaling pathway related molecules lead to the decline of lung function in children.It is speculated that the activation of IL-6/STAT3 signaling pathway might trigger the imbalance of Th17/Treg cells,which in turn affected the corresponding cytokine levels,leading to the occurrence and development of Mycoplasma pneumonia.

关 键 词：白细胞介素-6信号通路 信号转导子和转录激活子3信号通路 肺炎支原体肺炎 Th17细胞 TREG细胞 

分 类 号：R563.1[医药卫生—呼吸系统]