痰热清与头孢曲松钠联用对巨噬细胞极化的影响  

Effect of Tanreqing combined with ceftriaxone sodium on macrophage polarization

作　　者：刘晓霞[1] 陈剑华[1] 郭旭丽 李卫霞[1] 王雪玲[1] 熊南燕[1] LIU Xiaoxia;CHEN Jianhua;GUO Xuli;LI Weixia;WANG Xueling;XIONG Nanyan(Medical College of Hebei University of Engineering,Handan 056038,China)

机构地区：[1]河北工程大学医学院,邯郸056038

出　　处：《中国免疫学杂志》2023年第2期272-275,280,共5页Chinese Journal of Immunology

基　　金：河北省中医药管理局科研计划项目(2018155)。

摘　　要：目的:探讨痰热清与头孢曲松钠联用对金黄色葡萄球菌感染小鼠腹腔巨噬细胞极化的影响及机制。方法:小鼠分为对照组、模型组、头孢曲松钠组、痰热清组和痰热清+头孢曲松钠组,分别给予相应处理后,收集小鼠腹腔巨噬细胞。流式细胞术检测CD86/CD206表达;RT-qPCR和Western blot检测M1和M2型细胞标志物表达;Western blot检测IRF/STAT信号转导通路相关分子表达。结果:模型组、头孢曲松钠组、痰热清组、痰热清+头孢曲松钠组M1型巨噬细胞比例、标志物iNOS、TNF-α、IL-6、IL-1βmRNA和蛋白表达及p-STAT1、IRF5蛋白表达均显著高于对照组(P<0.05),痰热清组和痰热清+头孢曲松钠组显著高于模型组和头孢曲松钠组(P<0.05),但模型组与头孢曲松钠组差异无统计学意义(P>0.05),痰热清组与痰热清+头孢曲松钠组差异无统计学意义(P>0.05);各组M2型巨噬细胞比例、标志物Arg-1、Fizz-1、Ym-1、TGF-βmRNA及蛋白表达差异无统计学意义(P>0.05)。结论:痰热清与头孢曲松钠联用于金黄色葡萄球菌感染小鼠后,可通过促进M1型巨噬细胞分化,促进iNOS、TNF-α、IL-6、IL-1β表达,进而促进NO产生和炎症反应,增强机体抗感染能力,可能通过调节IRF/STAT信号转导途径实现。Objective:To discuss impact of Tanreqing combined with ceftriaxone sodium on macrophage polarization of mice infected with Staphylococcus aureus and its mechanism.Methods:Mice were divided into Control group,Model group,Ceftriaxone sodium group,Tanreqing group and Tanreqing+ceftriaxone sodium group.After corresponding treatment,mice peritoneal macrophages were collected.Flow cytometry was used to detected expressions of CD86 and CD206.RT-qPCR and Western blot were used to detected markers of M1 and M2 levels.Western blot was used to detected levels of moleculars of IRF/STAT signaling pathway.Results:Rate of M1 macrophage,mRNA and protein levels of iNOS,TNF-α,IL-6,IL-1β,and protein levels of p-STAT1 and IRF5 were obviously higher than control group(P<0.05),which were obviously higher in Tanreqing group and Tanreqing+ceftriaxone sodium group than model group and ceftriaxone sodium group(P<0.05),while there were no significance between model group and ceftriaxone sodium group(P>0.05),as well as Tanreqing group and Tanreqing+ceftriaxone sodium group(P>0.05).Rate of M2 macrophage,mRNA and protein levels of Arg-1,Fizz-1,Ym-1,TGF-βhad no significance in each group(P>0.05).Conclusion:Tanreqing combined with ceftriaxone sodium can promote into M1 macrophages differentiation in mice infected with Staphylococcus aureus,promote expressions of iNOS,TNF-α,IL-6 and IL-1β,enhance power of anti-infection via increasing amount of NO and inflammation,whose mechanism may regulating IRF/STAT signaling pathway.

关 键 词：痰热清 头孢曲松钠 巨噬细胞 极化 

分 类 号：R285.6[医药卫生—中药学] R392.12[医药卫生—中医学]