Plasma protein corona forming upon fullerene nanocomplex:Impact on both counterparts  

作　　者：Linjing Wu Fangqin Fu Wenhao Wang Wenhua Wang Zhengwei Huang Ying Huang Xin Pan Chuanbin Wu 

机构地区：[1]College of Pharmacy,Jinan University,Guangzhou,510632,China [2]School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou,510006,China

出　　处：《Particuology》2023年第2期26-36,共11页颗粒学报（英文版）

基　　金：supports from National Natural Science Foundation of China (grant Nos.82073774 and 82104070)。

摘　　要：Protein corona refers to the structure composed of biomolecules adsorbed on the surface of nanomaterials.The study on the effect of the interaction between protein and nanoparticles can provide an important guide for the application of nanodrug delivery.To provide a reference for the research on fullerene(C60)nanocomplex drug delivery systems,this work studied the interaction between C60 nanocomplex and a variety of plasma proteins.Research showed that the protein binding with C60 nanocomplex did not change the charge properties of protein.The proteins induced the aggregation of C60 nanocomplex.The circular dichroism spectra showed that the secondary structure of the proteins changed after binding to C60 nanocomplex.The ultraviolet-visible spectra showed that the effect of C60 nanocomplex on proteins was concentration-dependent.The fluorescence spectra showed that C60 nanocomplex could intrinsic fluorescence alteration of proteins.The adsorption capacity of C60 nanocomplex to proteins was changed at 0 h and 4 h.The interaction between nanocomplex and proteins might affect the morphological characteristics of nanocomplex and the conformation of proteins.This work could provide a reference for the research and development of C60 nanocomplex and other carbon-based nanocomplex as nanoparticulate drug delivery systems.

关 键 词：FULLERENE Nanocomplex INTERACTION Protein corona 

分 类 号：O62[理学—有机化学]