基于贴壁细胞应变场优化的硅胶小室研制  

Development of silica gel cell based on strain field optimization of adherent cells

作　　者：王紫琪 高丽兰[1,2] 吕林蔚[1,2] 张春秋[1,2] WANG Ziqi;GAO Lilan;LYU Linwei;ZHANG Chunqiu(Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control,Tianjin University of Technology,Tianjin 300384,China;National Demonstration Center for Experimental Mechanical and Electrical Engineering Education,Tianjin University of Technology,Tianjin 300384,China)

机构地区：[1]天津理工大学天津市先进机电系统设计与智能控制重点实验室,天津300384 [2]天津理工大学机电工程国家级实验教学示范中心,天津300384

出　　处：《天津理工大学学报》2022年第6期14-19,共6页Journal of Tianjin University of Technology

基　　金：国家自然科学基金资助项目(12072235);天津市科技计划项目生物医学工程科技重大专项(18ZXSGSY00010)。

摘　　要：细胞的生物力学研究正成为热点。目前,贴壁细胞拉压应变加载装置已在实验室中应用,但贴壁细胞应变加载的有效率存在缺陷。通过优化硅胶小室底面的厚度,通过有限元分析获得小室底面M形曲面的截面,通过制造模具和硅胶成型,研制了新型硅胶小室。结果表明:对于新型小室预期在1%,5%和10%应变场,应变均匀有效面积达到95%,93%和92%,较传统硅胶小室平板基底的应变场有效率分别提高了11.7%,65.9%和178.06%。优化后的硅胶小室厚度变化符合动态加载中柔性基底膜的均匀性要求,增大了贴壁细胞在应变载荷中的有效均匀面积,提高了应变加载的准确率,具有很高的实用价值。The biomechanical research of cells is becoming a hot spot. At present, tensile and compressive strain loading devices of adherent cells have been applied in laboratories, but the efficiency of adherent cell strain loading has defects. By optimizing the thickness of the bottom surface of the silica gel chamber, the section of the m-shaped surface of the bottom surface of the silica gel chamber is obtained by the finite element analysis, and then the new silica gel chamber is developed by manufacturing the mold and silica gel molding. The results show that the strain uniform effective area of the new cell reaches 95%, 93% and 92% when the strain field is expected to be 1%, 5% and 10%, which is 11.7%, 65.9% and 178.06% higher than that of the traditional silica gel cell plate base, respectively. The optimized thickness variation of silica gel cell conforms to the uniformity requirement of flexible basement membrane in dynamic loading, and increases the effective uniform area of adherent cells under strain loading. Therefore, the accuracy of strain loading is improved, and it has a high practical value.

关 键 词：贴壁细胞 应变均匀 硅胶小室 优化仿真 

分 类 号：R318.01[医药卫生—生物医学工程]