TBHQ对鸡舍PM_(2.5)诱导鸡胚肺组织细胞焦亡、坏死和炎症损伤的影响  

Effects of TBHQ on Pyroptosis,Necroptosis and Inflammatory Damage of Chicken Embryonic Lung Tissues Induced by PM_(2.5)from Chicken Houses

作　　者：郗蒙雪 沈丹 石一凡 李春梅[1] XI MengXue;SHEN Dan;SHI YiFan;LI ChunMei(College of Animal Science and Technology,Nanjing Agricultural University,Research Center for Livestock Environmental Control and Smart Production,Nanjing 210095)

机构地区：[1]南京农业大学动物科技学院,家畜环境控制与智慧生产研究中心,南京210095

出　　处：《中国农业科学》2023年第4期779-787,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31772648,32072781);江苏现代农业(肉鸡)产业技术体系环境控制创新团队(JATS[2022]480)。

摘　　要：【目的】探讨特丁基对苯二酚(tert-Butylhydroquinone,TBHQ)对鸡舍细颗粒物(fine particulate matter,PM_(2.5))诱导鸡胚肺损伤的缓解作用及机制,为预防和缓解鸡舍PM_(2.5)污染引起的鸡呼吸道健康问题提供理论依据。【方法】选用14日龄鸡胚作为研究对象,首先建立鸡舍PM_(2.5)诱导鸡胚肺组织损伤模型,再利用鸡胚肺损伤模型研究TBHQ的缓解作用。在建立鸡舍PM_(2.5)诱导鸡胚肺组织损伤模型试验中,选取不同浓度的PM_(2.5)(0、0.25、0.5、1 mg·mL^(-1))在14日龄鸡胚卵白处注射,5 d后,观察鸡胚存活率以及鸡胚肺组织形态,选取合适的PM_(2.5)处理浓度(0.25 mg·mL^(-1)),建立鸡胚肺损伤模型。在TBHQ对PM_(2.5)诱导鸡胚肺损伤的影响试验中,将14日龄鸡胚分为对照组(生理盐水)、PM_(2.5)组(0.25 mg·mL^(-1)PM_(2.5))、TBHQ组(0.1μg·mL^(-1)TBHQ)、PM_(2.5)+TBHQ组(0.25 mg·mL^(-1)PM_(2.5)+0.1μg·mL^(-1)TBHQ),分别注入鸡胚卵白处,试验持续5 d,采集肺组织,记录种蛋重量、胚胎重量以及肺组织重量;对鸡胚肺脏进行组织学观察;检测肺组织丙二醛(malondialdehyde,MDA)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、总抗氧化能力(total antioxidant capacity,T-AOC)和细胞焦亡(NLRP3、Caspase-1、IL^(-1)8、IL^(-1)β)以及细胞程序性坏死(RIPK1、RIPK3、MLKL)相关基因的表达。【结果】在建立鸡舍PM_(2.5)诱导鸡胚肺组织损伤模型试验中,不同浓度的PM_(2.5)(0、0.25、0.5、1 mg·mL^(-1))对鸡胚存活率无显著影响,但0.25mg·mL^(-1)PM_(2.5)组鸡胚肺组织出现炎性细胞浸润现象,0.5 mg·mL^(-1)和1 mg·mL^(-1)PM_(2.5)组鸡胚肺组织出现肺水肿现象,表明随着PM_(2.5)浓度增加,鸡胚肺部炎症损伤加重。在TBHQ对PM_(2.5)诱导鸡胚肺损伤的缓解试验中,各组对鸡胚胚胎重量、肺脏重量、胚蛋比和肺胚比均无影响;与PM_(2.5)组相比,TBHQ和PM_(2.5)共处理组的鸡胚肺组织中炎性细胞浸润现象明显减少,MDA�【Objective】The aim of this study was to investigate the protective effect and mechanism of tert-butylhydroquinone(TBHQ)on chicken embryo lung injury induced by fine particulate matter(PM_(2.5))in chicken house,so as to provide a theoretical basis for prevention and mitigation of chicken respiratory health problems caused by PM_(2.5)pollution in chicken house.【Method】14-day-old chicken embryos were selected as the research model in this study.Firstly,a model of lung tissue damage induced by PM_(2.5)from chicken houses was established,and then the alleviating effects of TBHQ on lung injury of chicken embryos was studied.In the experiment of establishing the model of chicken embryo lung tissue injury induced by PM_(2.5)from chicken houses,different concentrations of PM_(2.5)(0,0.25,0.5,and 1 mg·mL^(-1))were injected into egg albumens.After 5 days,the survival rate and lung tissues of chicken embryos were observed,and the appropriate concentration of PM_(2.5)(0.25 mg·mL^(-1))was selected to establish the model of chicken embryo lung injury.In the experiment of the effects of TBHQ on PM_(2.5)-induced lung injury of chicken embryos,chicken embryos were divided into control group(saline),PM_(2.5)group(0.25 mg·m L^(-1)PM_(2.5)),TBHQ group(0.1μg·mL^(-1)TBHQ)and PM_(2.5)+TBHQ group(0.25 mg·mL^(-1)PM_(2.5)+0.1μg·mL^(-1)TBHQ).The experiment lasted for 5 days.The lung tissues were collected and the egg weight,embryo weight and lung tissue weight were recorded.The expressions of MDA,T-SOD,T-AOC and pyroptosis-related genes(NLRP3,Caspase-1,IL^(-1)8,IL^(-1)β)and necroptosis-related genes(RIPK1,RIPK3,MLKL)in lung tissues of chicken embryos were detected.【Result】The results suggested that in the experiment to establish a model for PM_(2.5)-induced lung tissue damage in chicken embryos,different concentrations of PM_(2.5)(0,25,50,and 100μg·mL^(-1))from chicken houses did not significantly affect the survival of chicken embryos,but the lung tissues of chicken embryos in the 0.25 mg·mL^(-1)PM_(2.5)group showed

关 键 词：TBHQ 细胞焦亡 细胞程序性坏死 细颗粒物 鸡胚 氧化应激 

分 类 号：S831[农业科学—畜牧学]