4种呼吸道疫病血清学调查分析——以凯里市A奶牛场为例  

Serological Investigation and Analysis of Four Kinds of Respiratory Infectious Diseases-Taking a dairy farm in Kaili City as an example

作　　者：袁晓娟 吴冬梅 金鸿 倪定琴 姚曼 余琼 刘铭英 YUAN Xiaojuan;WU Dongmei;JIN Hong;NI Dingqin;YAO Man;YU Qiong;LIU Mingying(Qiandongnan Animal Disease Prevention and Control Center,Kaili Guizhou 556000;Kaili Animal Disease Prevention and Control Center,Kaili Guizhou 556000)

机构地区：[1]贵州省黔东南州动物疫病预防控制中心,贵州凯里556000 [2]贵州省凯里市动物疫病预防控制中心,贵州凯里556600

出　　处：《中国乳业》2023年第2期79-82,86,共5页China Dairy

摘　　要：[目的]为了解凯里市A奶牛场牛呼吸道疫病的流行情况。[方法]本次采用酶联免疫吸附试验(ELISA)对142份奶牛血清样品进行牛呼吸道合胞体病毒(BRSV)、牛病毒性腹泻(BVDV)、牛传染性鼻气管炎(IBRV)、牛支原体感染抗体检测。[结果]凯里市A奶牛场4种呼吸道疾病病原普遍存在,BRSV阳性率为41.5%(59/142);BVDV阳性率为40.1%(57/142);IBRV阳性率为45.0%(64/142);支原体阳性率为33.8%(48/142)。犊牛4种呼吸疾病单一感染率为15.1%~45.5%;BVDV感染最严重,为45.5%;成年牛单一感染率为33.8%~45.1%。此外,奶牛场存在IBRV、BRSV、BVDV、支原体多种病原体混合感染,二重感染率为18.3%~30.2%;BVDV+IBRV感染率最高,为30.2%;三重感染以BVDV+支原体+IBRV感染率最高,为19.7%;四重感染率为9.9%。[结论]凯里市A奶牛场存在4种疫病,有不同程度的病原体感染,应加强对上述病原体的流行控制,提高奶牛场管理水平,减少奶牛场疫病发生和经济损失。[Objective] To understand the epidemic situation of bovine respiratory disease in a dairy farm in Kaili City. [Methods] Bovine respiratory syncytial virus(BRSV),bovine viral diarrhea(BVDV),infectious bovine rhinotracheitis(IBRV) and bovine mycoplasma infection were detected in 142 serum samples by Elisa.[Results ] BRSV,BVDV,IBRV and Mycoplasma were common pathogens in a dairy farm in Kaili City. The positive rates of BRSV,BVDV, IBRV and Mycoplasma were 41.5%(59/142),40.1%(57/142),45.0%(64/142)and 33.8%(48/142)respectively. The single infection rate of four respiratory diseases in calves was 15.1% ~ 45.5%, BVDV infection was the most serious(45.5%),and the single infection rate of adult calves was 33.8%~45.1%. In addition,there were mixed infection of IBRV,BRSV,BVDV and Mycoplasma in dairy farm,the double infection rate was 18.3%~30.2%,BVDV + IBRV infection rate was the highest(30.2%),BVDV + Mycoplasma + IBRV infection rate was the highest,19.7%. The rate of quadruple infection was 9.9%. [Conclusion]There are four kinds of diseases in A Dairy Farm of Kaili City,and the pathogens are infected to different degrees. The epidemic control of the pathogens should be strengthened and the management level of the dairy farm should be improved,to reduce the incidence of disease and economic losses in dairy farms.

关 键 词：牛呼吸道疫病 血清学 调查 ELISA检测 多重感染 

分 类 号：S858.23[农业科学—临床兽医学]